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Species: Synthetic
Genetic Insert: W108 scRNA_MS2 and sgRNA_RR2
Vector Backbone Description: Vector Backbone:ColE1 vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29950558
Proper citation: RRID:Addgene_113326 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MS2 bacteriophage coat protein
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23073180
Proper citation: RRID:Addgene_113394 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty3-his3AI
Vector Backbone Description: Vector Backbone:YCplac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113393 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty3
Vector Backbone Description: Vector Backbone:YCplac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31249062
Proper citation: RRID:Addgene_113392 Copy
Genetic Insert: exo, beta, gam, sgRNA-FRT
Vector Backbone Description: Backbone Marker:Prather lab; Backbone Size:6959; Vector Backbone:pKDsgRNA-ack; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29884781
Comments: Must be used in combination with plasmid pCas9-CR4 (Addgene plasmid # 62655) to express Cas9.
Proper citation: RRID:Addgene_113398 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty3 Gag3 codon optimized for E. coli expression
Vector Backbone Description: Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_113397 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Red fluorescent protein, codon optimized for S. cerevisiae
Vector Backbone Description: Vector Backbone:pYES2.0; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113396 Copy
Species: Homo sapiens
Genetic Insert: Nbs1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28867292
Comments: Please note that 2 mutations in Gentamicin (C4S and K98R) were found during Addgene's quality control process. The depositing lab has noted that these 2 mutations do not affect Gentamicin selection.
Proper citation: RRID:Addgene_113312 Copy
Species: Synthetic
Genetic Insert: BCCP-PupE
Vector Backbone Description: Vector Backbone:pEF6a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104635
Proper citation: RRID:Addgene_113399 Copy
Species: Homo sapiens
Genetic Insert: QSOX1-enhancer
Vector Backbone Description: Vector Backbone:Tol2 reporter vector; Vector Types:Tol2 reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113383 Copy
Species: Homo sapiens
Genetic Insert: ESR1-enhancer
Vector Backbone Description: Vector Backbone:Tol2 reporter vector; Vector Types:Tol2 reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113382 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: Full length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11337 Copy
Species: Homo sapiens
Genetic Insert: PVT1-enhancer
Vector Backbone Description: Vector Backbone:Tol2 reporter vector; Vector Types:Tol2 reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113381 Copy
Species: Homo sapiens
Genetic Insert: human beta 1 ectodomain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6100; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28122868
Proper citation: RRID:Addgene_113387 Copy
Species: Mus musculus
Genetic Insert: pgk-1 5' and 3' regions
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3440520
Comments: Includes 500 bp mouse PGK promoter, 832 bp neo, 460 bp PGK 3' end.
Proper citation: RRID:Addgene_11333 Copy
Genetic Insert: 2 binding sites for let-7a miRNA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: 2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid.
Mutant binding site sequence is - TTACTTCA
Proper citation: RRID:Addgene_11332 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
Please note that Addgene's sequencing result differs from GenBank ID NM_067348, which indicates mutations in nud-1 are present at K136R, C220G and E235G.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11339 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty3-his3AI
Vector Backbone Description: Vector Backbone:pIBI20; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26421679
Proper citation: RRID:Addgene_113390 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11338 Copy
Species: Homo sapiens
Genetic Insert: rs281038 major allele
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29669022
Proper citation: RRID:Addgene_113371 Copy
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