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Genetic Insert: HTB tag
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pFA6ahphMX4; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26876 Copy
Genetic Insert: HTB tag
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pFA6akanMX6; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26875 Copy
Genetic Insert: CBFRE
Vector Backbone Description: Backbone Marker:Gaiano Lab; Backbone Size:0; Vector Backbone:CBFRE-EGFP; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: CBFRE: four (mutated) CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the mutated CBF1 binding element is (GATCTGGTGTAAA
CACGGGCTTGGAAAAAATTTATG).
Proper citation: RRID:Addgene_26870 Copy
Genetic Insert: HBH tag
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pFA6akanMX6; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26872 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:5609; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26966 Copy
Species: Homo sapiens
Genetic Insert: hChR2(E123T/H134R)-EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5587; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20081849
Comments: This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26968 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sgf73(1-106)
Vector Backbone Description: Backbone Size:5900; Vector Backbone:PET32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20395473
Proper citation: RRID:Addgene_26962 Copy
Genetic Insert: mir LEF1 1b
Vector Backbone Description: Backbone Size:6300; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19576624
Comments: shRNA sequence is 5'-GAGTTATTCCGGGTACATA
Proper citation: RRID:Addgene_27018 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sus1
Vector Backbone Description: Backbone Size:3669; Vector Backbone:PRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20395473
Proper citation: RRID:Addgene_26961 Copy
Genetic Insert: mir LEF1 3a
Vector Backbone Description: Backbone Size:6300; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19576624
Comments: shRNA sequence is 5'-GTTGCTGAGTGTACTCTAA
Proper citation: RRID:Addgene_27019 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sgf11
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:20395473
Comments: sgf 73(1-96) cloned in the second frame by NdeI+BglII for coexpression
Proper citation: RRID:Addgene_26964 Copy
Species: Homo sapiens
Genetic Insert: shRNA NEDD4L #1
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa
Proper citation: RRID:Addgene_27016 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sgf11
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:3620; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:20395473
Proper citation: RRID:Addgene_26960 Copy
Species: Homo sapiens
Genetic Insert: TAp63gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17399686
Proper citation: RRID:Addgene_26977 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26976 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4574; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26973 Copy
Species: Homo sapiens
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5386; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26975 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: cDNA PCRed from pCS2-Flag Smad3 with 5' primer containing Flag tag.
Proper citation: RRID:Addgene_27027 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7200; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: Fragment from pCS2-HA NEDD4L, fill-in to make blunt-end insert.
Proper citation: RRID:Addgene_27028 Copy
Species: Homo sapiens
Genetic Insert: LEF1
Vector Backbone Description: Backbone Marker:Addgene Plasmid 1764; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19576624
Proper citation: RRID:Addgene_27023 Copy
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