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Species: Other
Genetic Insert: LF82_535
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100349 Copy
Species: Other
Genetic Insert: LF82_434
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100346 Copy
Species: Other
Genetic Insert: LF82_572
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100352 Copy
Species: Other
Genetic Insert: LF82_606
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100356 Copy
Species: Other
Genetic Insert: LF82_619
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100357 Copy
Species: Other
Genetic Insert: LF82_250
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100329 Copy
Species: Homo sapiens
Genetic Insert: WDR92
Vector Backbone Description: Backbone Size:7800; Vector Backbone:PHAGE-P-CMVt-N-HA-GAW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25476789
Proper citation: RRID:Addgene_100161 Copy
Species: Homo sapiens
Genetic Insert: PRKAR2B
Vector Backbone Description: Backbone Size:7800; Vector Backbone:PHAGE-P-CMVt-N-HA-GAW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25476789
Proper citation: RRID:Addgene_100160 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100281 Copy
Species: Thermosynechococcus elongatus bp-1 for PcyA and HO1, and Synechocystis sp. PCC 6803 for Fd and Fnr.
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells.
Proper citation: RRID:Addgene_100280 Copy
Species: Thermosynechococcus elongatus bp-1 for PcyA and HO1, and Synechocystis sp. PCC 6803 for Fd and Fnr.
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to mouse BVRA is expressed so that PCB is further accumulated.
Proper citation: RRID:Addgene_100286 Copy
Species: Neisseria wadsworthii
Genetic Insert: Concentrative nucleoside transporter
Vector Backbone Description: Vector Backbone:pET26; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28424521
Proper citation: RRID:Addgene_100164 Copy
Species: Thermosynechococcus elongatus bp-1 for PcyA and HO1, and Synechocystis sp. PCC 6803 for Fd and Fnr.
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to human BVRA is expressed so that PCB is further accumulated.
Proper citation: RRID:Addgene_100285 Copy
Species: Homo sapiens
Genetic Insert: EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Proper citation: RRID:Addgene_100288 Copy
Species: Homo sapiens
Genetic Insert: EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Proper citation: RRID:Addgene_100287 Copy
Species: Other
Genetic Insert: LF82_662
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100326 Copy
Species: Other
Genetic Insert: LF82_240
Vector Backbone Description: Backbone Size:4502; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29762554
Proper citation: RRID:Addgene_100324 Copy
Species: Homo sapiens
Genetic Insert: 18 bp RFLP (MluI+KspAI)
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Proper citation: RRID:Addgene_100292 Copy
Species: Homo sapiens
Genetic Insert: 18 bp RFLP (MluI+KspAI)
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Human Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.
Proper citation: RRID:Addgene_100290 Copy
Species: Homo sapiens
Genetic Insert: PuroR.T2A.EGFP expression unit
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939824
Comments: Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al. 2017) for inducing homology-directed AAVS1 targeting. This donor plasmid has its targeting module flanked by the target site (TS) for gRNA.S1: 5’-CTGTCCCCTCCACCCCACAGTggggccactagggacaggattggTGACAGA-3’.
Proper citation: RRID:Addgene_100297 Copy
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