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https://www.addgene.org/pooled-library/chari-human-cell-surface-crispra/
Vector Backbone Description: Vector Types:CRISPR, Lentiviral, Mammalian Expression
Comments: FNLCR CRISPRa Cell Surface (Addgene #207471), FNLCR CRISPRa Soluble (Addgene #207472)
Proper citation: RRID:Addgene_1000000228 Copy
https://www.addgene.org/kits/ellis-myt/
Vector Backbone Description: Vector Types:Synthetic Biology
Comments: pMYT001 (Addgene #180654), pMYT002 (Addgene #180655), pMYT003 (Addgene #180656), pMYT004 (Addgene #180657), pMYT005 (Addgene #180658), pMYT006 (Addgene #203322), pMYT007 (Addgene #180660), pMYT008 (Addgene #180661), pMYT009 (Addgene #180662), pMYT010 (Addgene #180663), pMYT011 (Addgene #180664), pMYT012 (Addgene #180665), pMYT013 (Addgene #180666), pMYT014 (Addgene #180667), pMYT015 (Addgene #180668), pMYT016 (Addgene #180669), pMYT017 (Addgene #180670), pMYT018 (Addgene #180671), pMYT019 (Addgene #180672), pMYT020 (Addgene #180673), pMYT021 (Addgene #180674), pMYT022 (Addgene #180675), pMYT023 (Addgene #180676), pMYT024 (Addgene #180677), pMYT025 (Addgene #180678), pMYT026 (Addgene #180679), pMYT027 (Addgene #180680), pMYT028 (Addgene #180681), pMYT029 (Addgene #180682), pMYT030 (Addgene #180683), pMYT031 (Addgene #180684), pMYT032 (Addgene #180685), pMYT033 (Addgene #180686), pMYT034 (Addgene #180687), pMYT035 (Addgene #180688), pMYT036 (Addgene #180689), pMYT037 (Addgene #180690), pMYT038 (Addgene #180691), pMYT039 (Addgene #180692), pMYT040 (Addgene #180693), pMYT041 (Addgene #180694), pMYT042 (Addgene #180695), pMYT043 (Addgene #180696), pMYT044 (Addgene #180697), pMYT045 (Addgene #180698), pMYT046 (Addgene #180699), pMYT047 (Addgene #180700), pMYT048 (Addgene #180701), pMYT049 (Addgene #180702), pMYT050 (Addgene #180703), pMYT051 (Addgene #180704), pMYT052 (Addgene #180705), pMYT053 (Addgene #180706), pMYT054 (Addgene #180707), pMYT055 (Addgene #180708), pMYT056 (Addgene #180709), pMYT057 (Addgene #180710), pMYT058 (Addgene #180711), pMYT059 (Addgene #180712), pMYT060 (Addgene #180713), pMYT061 (Addgene #180714), pMYT062 (Addgene #180715), pMYT063 (Addgene #180716), pMYT064 (Addgene #180717), pMYT065 (Addgene #180718), pMYT066 (Addgene #180719), pMYT067 (Addgene #180720), pMYT068 (Addgene #180721), pMYT069 (Addgene #180722), pMYT070 (Addgene #180723), pMYT071 (Addgene #180724), pMYT072 (Addgene #180725), pMYT073 (Addgene #180726), pMYT074 (Addgene #180727), pMYT075 (Addgene #180728), pMYT076 (Addgene #180729), pMYT077 (Addgene #180730), pMYT078 (Addgene #180731), pMYT079 (Addgene #180732), pMYT080 (Addgene #180733), pMYT081 (Addgene #180734), pMYT082 (Addgene #198864), pMYT083 (Addgene #180736), pMYT084 (Addgene #180737), pMYT085 (Addgene #180738), pMYT086 (Addgene #180739), pMYT087 (Addgene #180740), pMYT088 (Addgene #180741), pMYT089 (Addgene #180742), pMYT090 (Addgene #180743), pMYT091 (Addgene #180744), pMYT092 (Addgene #198865), pMYT093 (Addgene #180746), pMYT094 (Addgene #180747), pMYT095 (Addgene #198866), pMYT096 (Addgene #198867)
Proper citation: RRID:Addgene_1000000229 Copy
https://www.addgene.org/pooled-library/reynolds-dhfr-mutagenesis/
Vector Backbone Description: Vector Types:Bacterial Expression
Comments: pTETDuet_SL1DHFR_R166QTS (Addgene #191274), pTETDuet_SL2DHFR_R166QTS (Addgene #191275), pTETDuet_SL3DHFR_R166QTS (Addgene #191276), pTETDuet_SL4DHFR_R166QTS (Addgene #191277)
Proper citation: RRID:Addgene_1000000195 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100091 Copy
Species: Homo sapiens
Genetic Insert: DNMT3A
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sgd1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
Proper citation: RRID:Addgene_100000 Copy
Species: Homo sapiens
Genetic Insert: SUVAR39H1
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100088 Copy
Species: Mus musculus
Genetic Insert: Ezh2[SET]
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100087 Copy
Species: Mus musculus
Genetic Insert: 14056
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100086 Copy
Species: Homo sapiens
Genetic Insert: FOG1(aa 1-45)
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100085 Copy
Species: Magnetococcus marinus
Genetic Insert: His-NavMs, Voltage gated sodium channel
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:6200; Vector Backbone:pTracer-CMV2, IRES GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28205548
Comments: Note: The GFP variant in this plasmid is missing the last five amino acids compared to the closest reference sequence (AAC53663.1). The depositor has confirmed that this does not impact plasmid function.
Proper citation: RRID:Addgene_100004 Copy
Species: Homo sapiens
Genetic Insert: mCerulean-P2A-putative cMYC DBD with a HA tag
Vector Backbone Description: Backbone Marker:Michel Sadelain (Addgene plasmid # 23244); Vector Backbone:pLM-mCerulean-cMYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28813671
Proper citation: RRID:Addgene_100125 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100002 Copy
Species: Homo sapiens
Genetic Insert: G9A
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28973434
Proper citation: RRID:Addgene_100089 Copy
Vector Backbone Description: Backbone Size:12919; Vector Backbone:pNOC-CRISPR-HA; Vector Types:Algae, Nannochloropsis expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29518315
Proper citation: RRID:Addgene_100008 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter 0.8 Kb Fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4322; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found two discrepancies compared to the depositor's sequence: 123_124insAATT, 162delG, & 854G>T. Some differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100129 Copy
Species: Caenorhabditis elegans
Genetic Insert: zif-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28619826
Proper citation: RRID:Addgene_100006 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter 4.8Kb Fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4334; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found the following discrepancies compared to the depositor's sequence: 367A>G, 739G>A, 928_929insA, 945delA, 1009C>T, 1682G>A, 2324A>T, 2503G>C, 3233A>G, 4092delG, 4784G>T. These differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100127 Copy
Species: Homo sapiens
Genetic Insert: miR-183-96-82 Cluster promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4334; Vector Backbone:pGL4; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28412746
Comments: Addgene's sequencing results found two discrepancies compared to the depositor's sequence: 555delG, 1247G>T. These differences could be SNPs and do not occur near the binding sites of the transcription factors of interest; thus they should not affect plasmid function.
Proper citation: RRID:Addgene_100126 Copy
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