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Species: Homo sapiens
Genetic Insert: Scambio
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESNEO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_33354 Copy
Species: Homo sapiens
Genetic Insert: MIB2
Vector Backbone Description: Vector Backbone:pCX-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_33312 Copy
Species: Homo sapiens
Genetic Insert: MIB1
Vector Backbone Description: Vector Backbone:pCX-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_33317 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report-LUC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: This is a translation reporter. KLF4 cDNA (an 306 bp EcoRI fragment derived from the KLF4 3' UTR) was cloned into the PmeI site in the 3'UTR of the firefly luciferase transgene in pMIR-Report-Luciferase. The insert/cDNA fragment was generated by cleavage at KLF4-internal EcoRI sites within the KLF4 3' UTR, followed by Klenow treatment.
Proper citation: RRID:Addgene_34598 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34593 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34589 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report-LUC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: This is a translation reporter. PCR primers were used to introduce mutations at the microRNA seed binding region of the K5S translational control element. The final constructs was confirmed by sequence analysis.
Proper citation: RRID:Addgene_34602 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report-LUC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: This is a translation reporter. PCR primers were used to introduce mutations at the microRNA seed binding region of the K5S translational control element. The final constructs was confirmed by sequence analysis.
Proper citation: RRID:Addgene_34601 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Marker:Morganstern JP and Land H 1990; Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: To prepare vector, a BamHI fragment encoding c-MYC was released from pBABE-puro-cMYC-ER. See Littlewood TD, Hancock DC, Danielian PS, Parker MG and
Evan GI. (1995). Nucleic Acids Res., 23, 1686–1690. A BamHI fragment encoding KLF4 was inserted into the same sites of this vector.
Proper citation: RRID:Addgene_34609 Copy
Species: Homo sapiens
Genetic Insert: PKM1
Vector Backbone Description: Backbone Marker:ATCC#87362 (PMID:7737504); Backbone Size:5557; Vector Backbone:P413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34607 Copy
Species: Homo sapiens
Genetic Insert: SENP3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_34554 Copy
Species: Homo sapiens
Genetic Insert: B23/nucleophosmin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4600; Vector Backbone:pCMV-DsRed-Express; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene quality control sequencing found an A123V point mutation, which, according to the depositing lab, should not alter the plasmid's function.
Proper citation: RRID:Addgene_34553 Copy
Species: Homo sapiens
Genetic Insert: L57
Vector Backbone Description: Backbone Marker:PMID: 12864922 ; Backbone Size:5000; Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: tandem fusion of LOV2 domain from A. Sativa phototropin 1, the catalytic domain of caspase-7 (human), and a mRFP variant that contains T140A and M141S mutations (introduced to facilitate cloning).
Proper citation: RRID:Addgene_34557 Copy
Species: Homo sapiens
Genetic Insert: SENP5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_34555 Copy
Species: Homo sapiens
Genetic Insert: pLyn-CaRQ
Vector Backbone Description: Backbone Marker:PMID: 12864922 ; Backbone Size:5000; Vector Backbone:pTriEx-3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: LYN membrane anchor - MGCIKSKGKDSATS
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL
Proper citation: RRID:Addgene_34559 Copy
Species: Homo sapiens
Genetic Insert: Wnt10B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_34748 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34695 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34694 Copy
Species: Homo sapiens
Genetic Insert: PRMT7
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34693 Copy
Species: Homo sapiens
Genetic Insert: TNKS2-M1054V
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_34692 Copy
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