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pCMX-Gal4-TEAD1-L272A
 
Resource Report
Resource Website 		RRID:Addgene_33037 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin L272A 2026-07-25 12:45:55 0
pCMX-Gal4-TEAD1-V391A
 
Resource Report
Resource Website 		RRID:Addgene_33038 TEAD1 Homo sapiens Ampicillin PMID:20123905 Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin V391A 2026-07-25 12:45:56 0
pCS2+MT-hToca-1-W518K
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33031 Toca-1 Homo sapiens Ampicillin PMID:15260990 Molecular cloning of Toca-1 was difficult, because the cDNA encoding human Toca-1 is lethal to E. coli. The toxicity problem was not resolved by using vectors with low copy numbers or with minimal transcriptional activity, suggesting that the source of toxicity was not the translated protein product but rather the DNA sequence itself. The toxic region of the Toca-1 cDNA was localized to codon 127. The Xenopus tropicalis homolog of Toca-1 is completely nontoxic when cloned using E. coli and is identical in amino acid sequence to human Toca-1 between amino acids 124 and 127, but these amino acids are encoded by different codons. Replacing codons 126 and 127 in the human Toca-1 with the X. Tropicalis sequence (which constitute silent mutations) completely ameliorated the toxicity problem and allowed us to propagate the human Toca-1 clone in E. coli without difficulty. Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin W518K 2026-07-25 12:45:55 1
pCS2+MT-hToca-1-wt
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33030 Toca-1 Homo sapiens Ampicillin PMID:15260990 Molecular cloning of Toca-1 was difficult, because the cDNA encoding human Toca-1 is lethal to E. coli. The toxicity problem was not resolved by using vectors with low copy numbers or with minimal transcriptional activity, suggesting that the source of toxicity was not the translated protein product but rather the DNA sequence itself. The toxic region of the Toca-1 cDNA was localized to codon 127. The Xenopus tropicalis homolog of Toca-1 is completely nontoxic when cloned using E. coli and is identical in amino acid sequence to human Toca-1 between amino acids 124 and 127, but these amino acids are encoded by different codons. Replacing codons 126 and 127 in the human Toca-1 with the X. Tropicalis sequence (which constitute silent mutations) completely ameliorated the toxicity problem and allowed us to propagate the human Toca-1 clone in E. coli without difficulty. Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:55 4
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
 
Resource Report
Resource Website 		RRID:Addgene_33255 PIK3CA Homo sapiens Ampicillin PMID:21876152 Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein" Backbone Size:5168; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin changed histidine 1047 to arginine and phenylalanine 486 to serine; deleted amino acids 343-346 2026-07-25 12:45:57 0
pBabe puro-HA-PIK3CA(H1047R;G320E)
 
Resource Report
Resource Website 		RRID:Addgene_33254 PIK3CA Homo sapiens Ampicillin PMID:21876152 Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein" Backbone Size:5168; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin changed histidine 1047 to arginine and glycine 320 to glutamic acid 2026-07-25 12:45:57 0
HT-ELK1
 
Resource Report
Resource Website 		RRID:Addgene_33258 ELK1 Homo sapiens Kanamycin Backbone Marker:Promega; Backbone Size:5051; Vector Backbone:pFC14K; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:57 0
pBabe puro-eIF4E
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33252 eIF4E Homo sapiens Ampicillin PMID:21876152 Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin unmodified 2026-07-25 12:45:57 2
pcDNA1 Flag ATXN1[85Q]-S776A
 
Resource Report
Resource Website 		RRID:Addgene_33240 Ataxin-1 Homo sapiens Ampicillin PMID:17190598 Backbone Marker:Invitrogen; Vector Backbone:pcDNA1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin insert contains a stretch of 85 Glutamines and no stop codon and a S776A mutation 2026-07-25 12:45:57 0
pcDNA1 Flag ATXN1[85Q]
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33237 Ataxin-1 Homo sapiens Ampicillin PMID:9353120 Backbone Marker:Invitrogen; Vector Backbone:pcDNA1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin insert contains a stretch of 85 Glutamines and no stop codon 2026-07-25 12:45:57 3
pcDNA1 Flag ATXN1[2Q]
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33235 Ataxin-1 Homo sapiens Ampicillin PMID:9353120 Backbone Marker:Invitrogen; Vector Backbone:pcDNA1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin insert contains 2Q (2 glutamines) and there isno stop codon 2026-07-25 12:45:57 1
pEGFP-ATXN1-2Q
 
Resource Report
Resource Website 		RRID:Addgene_33239 Ataxin-1 Homo sapiens Kanamycin PMID:9620770 Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin insert contains 2Q (2 glutamines) and there isno stop codon 2026-07-25 12:45:57 0
pCS260
 
Resource Report
Resource Website 		RRID:Addgene_33211 SYNM Homo sapiens Ampicillin PMID:21909094 Backbone Marker:Addgene plasmid #12177; Backbone Size:3745; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin miR-CGCG sites: aCgCgaa, aaCgCgaa 2026-07-25 12:45:57 0
pAG247
 
Resource Report
Resource Website 		RRID:Addgene_33200 LRIG1 Homo sapiens Ampicillin PMID:21909094 Backbone Marker:Addgene plasmid #12177; Backbone Size:3745; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-07-25 12:45:57 0
pCS247
 
Resource Report
Resource Website 		RRID:Addgene_33201 LRIG1 Homo sapiens Ampicillin PMID:21909094 Backbone Marker:Addgene plasmid #12177; Backbone Size:3745; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin miR-CGCG sites: aCgCgaa, aaCgCgaa 2026-07-25 12:45:57 0
pAG253
 
Resource Report
Resource Website 		RRID:Addgene_33208 RAP1A Homo sapiens Ampicillin PMID:21909094 Backbone Marker:Addgene plasmid #12177; Backbone Size:3745; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-07-25 12:45:57 0
pCS252
 
Resource Report
Resource Website 		RRID:Addgene_33207 MAP4K4 3'UTR Homo sapiens Ampicillin PMID:21909094 Backbone Marker:Addgene plasmid #12177; Backbone Size:3745; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin miR-CGCG sites: aaCgCga, aaCgCgaa 2026-07-25 12:45:57 0
pBG100-TCPTP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33365 TCPTP-45 Homo sapiens Kanamycin PMID:20940300 Backbone Marker:Vanberbilt structural biology core plasmid; Vector Backbone:pBG100; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-07-25 12:45:58 2
pIRES-Puro-TCPTP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33366 TCPTP-45 Homo sapiens Ampicillin PMID:20940300 Backbone Marker:Clontech; Vector Backbone:pIRESpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:58 1
pGex-Cav1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_33364 Cav1 Homo sapiens Ampicillin PMID:20940300 Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:58 2

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