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Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160206 Copy
Vector Backbone Description: Backbone Marker:Partially Addgene; Backbone Size:5336; Vector Backbone:Constructed from MoClo Yeast Toolkit (Kit # 1000000061) and own yeast homology arms (Addgene plasmids 125032 and 125065); Vector Types:Yeast Expression, Kluyveromyces marxianus; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33176787
Proper citation: RRID:Addgene_160207 Copy
Vector Backbone Description: Backbone Marker:Dianne Duncan; Backbone Size:1961; Vector Backbone:pXZ13lacZalpha; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modification of pXZ13lacZalpha construct to exchange Kanamycin gene for original Ampicillin gene. This improves upon the cloning efficiency of the original pXZ13 vector (reference below) in three ways: 1) The distance between the Esp3I sites is increased to increase cutting efficiency, 2) lacZalpha complementation is introduced to allow for blue/white screening of recombinants, and 3) Kanamycin selection removes 3 of the 4 original parental plasmids from forming colonies in the final plating.
Original vector reference:
Zhang X, Koolhaas WH, Schnorrer F. A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3 (Bethesda). 2014 Oct 15;4(12):2409-18
Proper citation: RRID:Addgene_160230 Copy
Species: Mus musculus
Genetic Insert: Ifih1
Vector Backbone Description: Vector Backbone:pLenti6.2 backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160110 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pLenti6.2 backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160111 Copy
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30083222
Proper citation: RRID:Addgene_160112 Copy
Species: Other
Genetic Insert: Tetracycline repressor
Vector Backbone Description: Backbone Size:7600; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33963794
Proper citation: RRID:Addgene_160235 Copy
Genetic Insert: Ttg Operator (2x)
Vector Backbone Description: Backbone Size:7200; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33963794
Proper citation: RRID:Addgene_160238 Copy
Genetic Insert: Pip Operator (3x)
Vector Backbone Description: Backbone Size:7400; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33963794
Proper citation: RRID:Addgene_160239 Copy
Vector Backbone Description: Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160185 Copy
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160186 Copy
Species: Mus musculus
Genetic Insert: Mavs
Vector Backbone Description: Vector Backbone:pLX304 backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160100 Copy
Species: Mus musculus
Genetic Insert: Fbxw7 alpha R505C
Vector Backbone Description: Vector Backbone:pENTR1A backbone; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160103 Copy
Species: Mus musculus
Genetic Insert: Fbxw7 alpha R505C
Vector Backbone Description: Vector Backbone:pLX304 backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160104 Copy
Species: Mus musculus
Genetic Insert: Fbxw7alpha
Vector Backbone Description: Vector Backbone:pLX304 backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160106 Copy
Species: Mus musculus
Genetic Insert: Ddx58
Vector Backbone Description: Vector Backbone:pENTR223.1 backbone; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160107 Copy
Vector Backbone Description: Backbone Marker:Zhang et al. G3. 2014 Oct 15;4(12):2409-18; Backbone Size:2528; Vector Backbone:pXZ13 (Addgene plasmid #60949); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This vector is an improvement upon the published pXZ13 vector for Golden Gate cloning of the donor plasmid in the published article noted below. The vector increases cloning efficiency in two ways: 1) the distance between the Esp3I sites is increased to insure complete cutting at both sites, and 2) lacZ alpha is placed between the two cutting sites to provide Blue/White screening of recombinants.
Vector progenitor reference:
Zhang X, Koolhaas WH, Schnorrer F. A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. G3(Bethesda). 2014 Oct 15;4(12):2409-18.
Proper citation: RRID:Addgene_160229 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pLX304 backbone; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160095 Copy
Species: Mus musculus
Genetic Insert: Irf1
Vector Backbone Description: Backbone Size:3811; Vector Backbone:pDONR221 backbone; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160097 Copy
Species: Mus musculus
Genetic Insert: Mavs
Vector Backbone Description: Vector Backbone:pENTR1A backbone; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32371478
Proper citation: RRID:Addgene_160099 Copy
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