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Species: Lachancea fermentati
Genetic Insert: mfalpha1Lf
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176717 Copy
Species: Kazachstania naganishii
Genetic Insert: mfalpha1Kn
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176716 Copy
Species:
Genetic Insert: Cre-P2A-tdTomato
Vector Backbone Description: Vector Backbone:AAVS1-tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.24.485641v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176837 Copy
Species: Lachancea thermotolerans
Genetic Insert: mfalpha1Lt
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176719 Copy
Species: Lachancea mirantina
Genetic Insert: mfalpha1Lm
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The gene was codon-optimized for expression in S. cervisiae and cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176718 Copy
Species: Homo sapiens
Genetic Insert: Human CYP3A4 proximal enhancer sgRNA for activation
Vector Backbone Description: Vector Backbone:lenti-sgRNA(MS2)_zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.24.485641v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176839 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-PRM1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176709 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: RSP23
Vector Backbone Description: Backbone Size:4886; Vector Backbone:pLIB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176702 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-MSG5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: The part was cloned into the vector using Goden Gate. The plasmid is Golden Gate Cloning compatible. Please visit https://doi.org/10.1101/2021.09.27.462023 for bioRxiv preprint.
Proper citation: RRID:Addgene_176708 Copy
Species: Homo sapiens
Genetic Insert: DOP1B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: DOP1B (BC156374.1) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176979 Copy
Species: Mus musculus
Genetic Insert: Rcan1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Rcan1 (NM_019466.4) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176978 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGCACGTCGTATGGGTACTTGAAGT- TGGCATAATCTGA-3'. The 5' primer contains a HindIII site, a Kozak consensus sequence CCACC and the start codon. The 3' primer contains the coding sequence for the HA epitope YPYDVPDYA, the stop codon and a HindIII site. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk (Life Technologies), it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17691 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha CCSS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17692 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha Y789F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: PTPalpha(Y789F)-HA was generated using pXJ41PTPalpha as a template with the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGGCACGTCGTATGGGTACTTGAAGTTGGCAAAATCTGA-3'. The substitution ATA->AAA results in a Tyr789->Phe substitution. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17693 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha S204A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17694 Copy
Species: Homo sapiens
Genetic Insert: MOSMO
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: MOSMO (NM_001164579.2) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176975 Copy
Species: Homo sapiens
Genetic Insert: RUNX1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: RUNX1 (BC136381) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176974 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha S204A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17699 Copy
Species: Homo sapiens
Genetic Insert: GLI1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GLI1 (NM_005269.2) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176968 Copy
Species: Homo sapiens
Genetic Insert: CHAF1B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: CHAF1B (NM_005441) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176969 Copy
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