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Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20907 Copy
Species: Mus musculus
Genetic Insert: KLF4
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20906 Copy
Species: Mus musculus
Genetic Insert: MyoD~E12 forced dimer
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20912 Copy
Species: Mus musculus
Genetic Insert: Inhibitor of DNA binding 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20965 Copy
Species: Mus musculus
Genetic Insert: Inhibitor of DNA binding 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20964 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene has detected several mutations in the UTR region of this construct.
Proper citation: RRID:Addgene_20977 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Vector Backbone:MSCV (murine stem cell virus); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: *Please note that the full sequence verified by Addgene differs from the original depositor's map and thus the numbers and nomenclature used by the depositor may differ from the Addgene map.*
Depositor's Comments:
We discovered that, although a 1,975-nt full-length Hoxa9 clone (numbered from the protein start site; I.M.A.G.E. clone BC055059) would produce protein when transiently expressed from the murine stem cell virus (MSCV) long terminal repeat, following retroviral transduction, the HOXA9 protein could not be detected. Since this cDNA contained two putative polyadenylation signals in the 3' UTR, we removed these sites to determine if they were interfering with retrovirally mediated protein expression. The site at nt 1798 was replaced using a primer containing a KpnI sequence in place of the AATAAA sequence together with a primer containing the BglII site at nt 749 to amplify a 1,052-nt BglII-KpnI fragment by PCR. A second primer containing the KpnI site in place of the polyadenylation signal at nt 1798 was used in conjunction with a primer containing an AAT-to-CGG change in the polyadenylation signal at nt 1975 and an adjacent XhoI site for cloning to generate a 150-nt KpnI-XhoI fragment. Standard methods were used to combine these fragments to create a 1,975-nt Hoxa9 cDNA encoding an N-terminal FLAG-tagged version of the mature protein but lacking the two putative polyadenylation signals. The resulting MSCV clone produced high levels of FLAG-HOXA9 protein and immortalized BM cells.
Proper citation: RRID:Addgene_20975 Copy
Species: Mus musculus
Genetic Insert: cMyc, KLF4, Oct4, Sox2
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments: Additional reference, Kaji et al Nature. 2009 Mar 1
Proper citation: RRID:Addgene_20959 Copy
Species: Mus musculus
Genetic Insert: cMyc
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20888 Copy
Species: Mus musculus
Genetic Insert: p21 3'UTR site 1 and 2 mutation
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20880 Copy
Species: Mus musculus
Genetic Insert: Rb 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: One C->A mutation is present but does affect function of plasmid.
Proper citation: RRID:Addgene_20883 Copy
Species: Mus musculus
Genetic Insert: HOXB9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20997 Copy
Species: Mus musculus
Genetic Insert: PRRX2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21011 Copy
Species: Mus musculus
Genetic Insert: PRRX2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21010 Copy
Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The protein product of this clone exhibits good HAT activity on histone substrates.
Proper citation: RRID:Addgene_21093 Copy
Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP65; Vector Types:in vitro TNT; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21092 Copy
Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21091 Copy
Species: Mus musculus
Genetic Insert: MEIS1B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21022 Copy
Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP65; Vector Types:in vitro TNT; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21026 Copy
Species: Mus musculus
Genetic Insert: MSX2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5376; Vector Backbone:pET-28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21025 Copy
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