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Plasmids are provided by Addgene and DGRC.

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pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_30505 beta-glucuronidase Escherichia coli Ampicillin PMID:22020504 In our experience, plasmid yields are highest when cells are harvest at late log stage. Note that this plasmid also confers resistance to Spectinomycin, but Addgene provides it in an Amp stab. Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:45:38 2
pET21a-AcrR
 
Resource Report
Resource Website 		RRID:Addgene_51148 AcrR Escherichia coli Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-07-25 12:48:10 0
pET21a-BetI
 
Resource Report
Resource Website 		RRID:Addgene_51156 BetI Escherichia coli Ampicillin PMID:24316737 UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector. Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-07-25 12:48:10 0
pBAD-LacI
 
Resource Report
Resource Website 		RRID:Addgene_46394 LacI Escherichia coli Ampicillin PMID:23834731 LacI expression was induced with 0.2% (w/v) L-arabinose and cells incubated for 4 h at 20 °C. Backbone Marker:Invitrogen; Backbone Size:4092; Vector Backbone:pBADmyc-hisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin silent T to C mutation at position 19 2026-07-25 12:47:33 0
pRF-BetI
 
Resource Report
Resource Website 		RRID:Addgene_49357 BetI repressor Escherichia coli Ampicillin PMID:24316737 Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor). Vector Backbone:pEXT20; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-07-25 12:47:57 0
pGEMABC
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_45398 rpoA Escherichia coli Ampicillin PMID:23389035 Backbone Marker:Promega; Backbone Size:3993; Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:47:26 6
pZJQIBEBT003
 
Resource Report
Resource Website 		RRID:Addgene_140053 eMutS-L157C-G233C-CBM-EGFP Escherichia coli Ampicillin PMID:32135061 Vector Backbone:pET21c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin L157C, G233C 2026-07-25 12:39:31 0
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_140018 EcoTyrRS Escherichia coli Ampicillin PMID:37935196 Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Y37L, D182S, F183M, L186A 2026-07-25 12:39:30 2
pET15b-His-RF2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124130 Release factor 2 Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pQE60-RRF-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124132 Ribosome recycling factor Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pET21a-MTF-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124133 Methionyl-tRNA formyltransferase Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pET21a-TyrRS-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124121 Tyrosyl-tRNA ligase Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pET21a-TrpRS-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124120 Tryptophanyl-tRNA ligase Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pET21a-ValRS-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124122 Valyl-tRNA ligase Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pQE30-His-IF3
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124125 Initiation factor 3 Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pQE60-EFTu-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124127 Elongation factor Tu Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pQE60-EFG-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124126 Elongation factor G Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pQE60-EFTs-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124128 Elongation factor Ts Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pET21a-GlyRS-His
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124110 Glycyl-tRNA ligase Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 1
pET21a-His-IleRS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_124112 Isoleucyl-tRNA ligase Escherichia coli Ampicillin PMID:11479568 It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region. Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:37:10 2

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