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Species: E. coli
Genetic Insert: mcherry
Vector Backbone Description: Vector Backbone:pOSIP-KL; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_116947 Copy
Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100916 Copy
Species: E. coli
Genetic Insert: Ptrc promoter driving GalP transporter expression
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat)
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101843 Copy
Species: E. coli
Genetic Insert: Formaldehyde repressor protein (frmR)
Vector Backbone Description: Vector Backbone:pTR47m4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28921510
Proper citation: RRID:Addgene_102436 Copy
Species: E. coli
Genetic Insert: sgGal4
Vector Backbone Description: Backbone Marker:Addgene #51024; Vector Backbone:pSLQ1651-sgRNA(F+E); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841410
Proper citation: RRID:Addgene_100549 Copy
Species: E. coli
Genetic Insert: Lvl0 5'-2xTetApt-mVenus-PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115962 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR SapI/BsaI Mut
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115947 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65608 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65569 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65601 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65604 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65603 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65579 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65576 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65574 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65581 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65589 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65582 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26157619
Proper citation: RRID:Addgene_65585 Copy
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