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Species: Synthetic
Genetic Insert: PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: SC101
Proper citation: RRID:Addgene_107900 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT2 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_108444 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_108441 Copy
Species: Arabidopsis thaliana
Genetic Insert: GA2ox2
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_108567 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT2
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_108566 Copy
Species: Arabidopsis thaliana
Genetic Insert: GA2ox2 promoter
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_108564 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMpGE010; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_108681 Copy
Species: Synthetic
Genetic Insert: Atco-Cas9-Pea3ter
Vector Backbone Description: Backbone Marker:Kohchi Lab., Kyoto; Backbone Size:11315; Vector Backbone:pMpGWB103; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_108722 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Fn; Vector Types:Plant Expression, CRISPR, Gateway compatible crRNA entry clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_108864 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible Cpf1 entry clone; Bacterial Resistance:Spectinomycin
References:
Comments: Cpf1 is rice codon optimized
Proper citation: RRID:Addgene_108859 Copy
Species: Homo sapiens
Genetic Insert: human full-length REST/NRSF
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pIDS; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_109162 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_109234 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_109237 Copy
Species:
Genetic Insert: g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_115484 Copy
Species:
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Species: Arabidopsis thaliana
Genetic Insert: pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_115995 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116013 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116016 Copy
Species: Arabidopsis thaliana
Genetic Insert: pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116010 Copy
Species: Arabidopsis thaliana
Genetic Insert: pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116012 Copy
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