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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5993; Vector Backbone:pDE43-MCK; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49523 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2051; Vector Backbone:pENTR1A; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme. It serves to create intermediate supermodules with plant resistance cassettes.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 358-368bp
BsaI site #2 = 1796-1806bp
Proper citation: RRID:Addgene_48865 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124245 Copy
Species: Synthetic
Genetic Insert: catP/sfGFP
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pGT-Ah7; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: See supplemental tables of the referenced paper for more information on each plasmid.
Proper citation: RRID:Addgene_122574 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag.
Proper citation: RRID:Addgene_18671 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:13430; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098
Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_174050 Copy
Species: Spinacia oleracea
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
Proper citation: RRID:Addgene_126054 Copy
Species: Spinacia oleracea
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
Proper citation: RRID:Addgene_126055 Copy
Species: Synthetic
Genetic Insert: CAMKIIA promoter and C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_128461 Copy
Species: Synthetic
Genetic Insert: C-terminal V5 tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_128440 Copy
Species: Synthetic
Genetic Insert: Ef1a promoter, N-terminal HA tag, and SV40 promoter
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_128446 Copy
Species: Synthetic
Genetic Insert: C-terminal HA tag
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc; Backbone Size:4796; Vector Backbone:pSMPUW-eMCS; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_128439 Copy
Species: Synthetic
Genetic Insert: Rhizobium codon-optimized ABEs
Vector Backbone Description: Vector Backbone:pKm; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170332 Copy
Species: Synthetic
Genetic Insert: Rhizobium codon-optimized CBEs
Vector Backbone Description: Vector Backbone:pKm; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170333 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167673 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167672 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167671 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_161893 Copy
Species: Synthetic
Genetic Insert: pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
Vector Backbone Description: Backbone Marker:Christopher Voigt's Lab; Vector Backbone:pTHSSd_1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_161937 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUBQ10-GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://doi.org/10.21203/rs.3.rs-3558280/v1 for preprint.
Proper citation: RRID:Addgene_209351 Copy
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