Searching the RRID Resource Information Network
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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: 8xHis-mCherry-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67139 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: 8xHis-eGFP-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67137 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: 8xHis-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67135 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: attR1-ccdB-CamR-attR2-8xHis-Stop
Proper citation: RRID:Addgene_67136 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCellFree_G05; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: sfGFP
Proper citation: RRID:Addgene_67143 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: attR1-ccdB-CamR-attR2-mCheery-8xHis-Stop
Proper citation: RRID:Addgene_67140 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Myc-8xHis-mCherry-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67141 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67618 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67619 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67711 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pmTol2; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67745 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8658; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61600 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7988; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61702 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_62179 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:John Doench; Backbone Size:9240; Vector Backbone:pcw107; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_62511 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59382 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat2
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59385 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Gateway cassette was inserted between SacI and XhoI restriction sites in the MCS.
Proper citation: RRID:Addgene_60323 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pPB-PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60436 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7105; Vector Backbone:pDEST-0GWA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60556 Copy
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