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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGC480
 
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RRID:Addgene_42600 let-363 cDNA Caenorhabditis elegans Ampicillin and Tetracycline PMID:22278922 This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain. Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:47:02 0
pGC479
 
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RRID:Addgene_42601 daf-15 Caenorhabditis elegans Ampicillin and Tetracycline PMID:22278922 This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain. Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known. Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:47:02 0
q4wt
 
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RRID:Addgene_24502 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with an HA epitope engineered into an internal site that does not seem to affect receptor coupling in multiple studies. Epitope tagged by Paul Wilson, see Wedegaertner, JBC 268: 25001-25008. The 5' non-coding sequences were removed, but the 3' non-coding sequences remain, as in Strathmann & Simon (1990) PNAS 87:9113-9117. The parent construct has been donated to the ATCC by the Bourne Lab. There is a 1bp insertion, a 5bp insertion, and a 1bp deletion when author sequence is aligned with Addgene quality control sequence. These differences are between the stop codon and the SP6 sequence. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:44:50 0
pZV_21_400
 
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RRID:Addgene_170477 T7A1, O2, O1, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline PMID:28295806 LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pRS_2S_400
 
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RRID:Addgene_170478 T7A1, O2, Os, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pYY_N1_400
 
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RRID:Addgene_170473 T7A1, O-, O1, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pYY_S2_400
 
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RRID:Addgene_170471 T7A1, Os, O2, Lambda t1 in lacZ fragment Synthetic Ampicillin and Tetracycline LacI operators separated by 400 base pairs. Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:42:37 0
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST
 
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RRID:Addgene_182547 H5_Vietnam_1203_04_Y161A Ampicillin and Tetracycline PMID:31189111 Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Y161A mutant that confers exclusive NeuAc to NeuGc specificity 2026-07-25 12:54:31 0
pPtPBR1
 
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RRID:Addgene_80388 Ampicillin and Tetracycline Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:52:21 0
Human p150,95
 
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1+ mentions
RRID:Addgene_8633 Human p150,95 Homo sapiens Ampicillin and Tetracycline PMID:3327687 This clone will direct the expression of functional p150,95 upon cotransfection with the beta2 subunit in a variety of cell types. This sequence has been deposited in Genbank with accession number Y00093. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991). Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:53:11 2
pAXEF-Rap1-WT
 
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1+ mentions
RRID:Addgene_32697 RAP1 Homo sapiens Ampicillin and Tetracycline PMID:15469846 Backbone Marker:Anumanthan et al. 1998; Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:45:53 1
qz5
 
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RRID:Addgene_25867 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gz alpha(YIGLC) 2026-07-25 12:45:02 0
pCDM8-GlyCAM-1/IgG
 
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RRID:Addgene_17841 GlyCAM-1/IgG Mus musculus Ampicillin and Tetracycline PMID:10330415 Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:43:39 0
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST
 
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RRID:Addgene_175020 D/bovine/Nebraska/9-5/2012 Synthetic Ampicillin and Tetracycline PMID:33807137 Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline s57a esterase knock out mutant 2026-07-25 12:43:10 0
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST
 
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RRID:Addgene_175019 D/bovine/Nebraska/9-5/2012 Synthetic Ampicillin and Tetracycline PMID:33807137 Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:43:10 0
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
 
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RRID:Addgene_107582 GFP+ Synthetic Ampicillin and Tetracycline PMID:29666238 Backbone Marker:N/A; Backbone Size:5796; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline F64L/ S65T/ Q80R/ F99S/ M153T/ V163A 2026-07-25 12:34:33 0
pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015
 
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RRID:Addgene_107578 GFP+ Synthetic Ampicillin and Tetracycline PMID:29666238 Backbone Marker:N/A; Backbone Size:6336; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline F64L/ S65T/ Q80R/ F99S/ M153T/ V163A 2026-07-25 12:34:33 0
pFLP3
 
Resource Report
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1+ mentions
RRID:Addgene_64946 Flp recombinase Ampicillin and Tetracycline PMID:15908923 GenBank Accession # AY597273 Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown. Backbone Marker:Herbert Schweizer; Vector Backbone:pFLP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:50:04 4
pFTC1
 
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RRID:Addgene_64951 FRT-TcR-FRT Ampicillin and Tetracycline PMID:15908923 GenBank Accession #AY712950 Compared to the Genbank reference sequence AY712950, Addgene's sequencing results found a T350I mutation in the tetracycline cassette. The effect of this difference is unknown. Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline 2026-07-25 12:50:04 0
mCHOP10(dLZ).pCDNA1
 
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RRID:Addgene_21900 CHOP10 Mus musculus Ampicillin and Tetracycline PMID:1547942 Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133. Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline The coding region of the murine CHOP-10 cDNA was interrupted at the unique Nhe1 site, making delta leucine zipper 2026-07-25 12:44:28 0

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