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Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120425 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3, IS150
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120424 Copy
Species: Escherichia coli
Genetic Insert: AraC
Vector Backbone Description: Backbone Size:2147; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please note that pSB1A2 was used to create the plasmid rather than pSB1A3. This does not affect function as described in the associated publication.
Proper citation: RRID:Addgene_116850 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 22-30
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Kanamycin
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
Proper citation: RRID:Addgene_117762 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 14-21
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Kanamycin
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
Proper citation: RRID:Addgene_117761 Copy
Species: Escherichia coli
Genetic Insert: Translation factor cistrons 1-13
Vector Backbone Description: Backbone Size:5018; Vector Backbone:BioBrick pET-24a(+); Vector Types:Escherichia coli; Bacterial Resistance:Tetracyline and Kanamycin
References:
Comments: The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding.
Proper citation: RRID:Addgene_117760 Copy
Species: Escherichia coli
Genetic Insert: BetI repressor
Vector Backbone Description: Vector Backbone:pEXT20; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor).
Proper citation: RRID:Addgene_49357 Copy
Species: Escherichia coli
Genetic Insert: AcrR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51148 Copy
Species: Escherichia coli
Genetic Insert: BetI
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51156 Copy
Species: Escherichia coli
Genetic Insert: gusA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Matsumura lab strain number 337
Proper citation: RRID:Addgene_61156 Copy
Species: Escherichia coli
Genetic Insert: RNase HI-D10R-E48R
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60367 Copy
Species: Escherichia coli
Genetic Insert: RNase HI
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60365 Copy
Species: Escherichia coli
Genetic Insert: YajL
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60716 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_55749 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_55751 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_55750 Copy
Species: Escherichia coli
Genetic Insert: hygromycin B phosphotransferase
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Proper citation: RRID:Addgene_58223 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: AUG --> UUG start codon in lacZ
Proper citation: RRID:Addgene_63861 Copy
Species: Escherichia coli
Genetic Insert: e. coli galactokinase
Vector Backbone Description: Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_26958 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a -1 frameshift mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63875 Copy
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