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Species:
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pCAH01SpR; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Please visit https://doi.org/10.1101/2025.05.28.656465 for bioRxiv preprint.
Proper citation: RRID:Addgene_251729 Copy
Species: Other
Genetic Insert: aadA gene flanked by flv3 upstream and downstream regions
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2986; Vector Backbone:pGEM®-T easy; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_185533 Copy
Species: Synechoccus elongatus PCC 7942
Genetic Insert: YFP-KaiC
Vector Backbone Description: Vector Backbone:Ptrc-yfp-kaiC expressed from NS1 vector with SpSm resistance constructed by seamless assembly.; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_85107 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli with point mutation E335Q
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_100917 Copy
Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431E T432E with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_101836 Copy
Species: NA
Genetic Insert: sp/st resistance casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_101846 Copy
Species: S. elongatus PCC7942
Genetic Insert: RpaA with its native promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_101829 Copy
Species: Synthetic
Genetic Insert: CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
Vector Backbone Description: Backbone Marker:Andrew Gleave; Backbone Size:11612; Vector Backbone:pART27; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: pART27::CLOMELEON is a binary pART27 vector harbouring a NotI-fragment with a gene cassette subcloned from pART7::CLOMELEON to stably insert the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) into the genome of plants by Agrobacterium-mediated gene transfer, e.g. by floral dip (Clough & Bent 1998; PubMed: 10069079; doi: 10.1007/BF00028910). See the Supplemental Document under Resource Information for more details, including annotated maps.
Proper citation: RRID:Addgene_247988 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pDFC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_251363 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMCY2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Two cloning steps are required for the generation of a "promoter:3xHA:VENUS:GENE:2A:mTurqN7" fragment:
A) Cloning of promoter:
Primer design:
Forward Primer: 5’GATCGGGGCGCGCCCTCGAG+PROMOTER SPECIFIC SEQUENCE 3’
Reverse primer: 5’ GATGGGCATTAACATCTTTTAC+PROMOTER SPECIFIC SEQUENCE 3'
Digest plasmid with SapI
Clean
Perform Gibson Assembly
Transform E. coli and select positive colonies LB+Sp/Sm (blue/white selection)
B) Cloning of gene fragment:
Forward Primer: 5’GTTCTGGTGGGGGTGGCTCC+ GENE SPECIFIC SEQUENCE (starting from the ATG) 3’
Reverse primer: 5’ AACAGCTGGCCCGAGCCACC+GENE SPECIFIC SEQUENCE (without STOP Codon) 3'
Digest plasmid with Bsu36I and perform gel purification
Perform Gibson Assembly
Transform E. coli and select positive colonies LB+Sp/Sm
The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_239459 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMCY2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: For cloning a promoter + gene fragment (without stop codon) via Gibson Assembly, we recommend the following approach:
Primer design:
Forward primer: 5’GATCGGGGCGCGCCCTCGAG+PROMOTER SPECIFIC PRIMER 3’
Reverse primer: 5’ CAGAACCACCACCTCCGGATCC+GENE SPECIFIC PRIMER W/O STOP CODON 3'
Digest pMDS1 plasmid with SAP I enzyme
Clean up
Perform Gibson assembly reaction
Transform E. coli (DH5alpha)
Select on LB + Sp + Sm
Proper citation: RRID:Addgene_239460 Copy
Species: Mus musculus
Genetic Insert: Dishevelled-2
Vector Backbone Description: Backbone Marker:Novagen/EMD Biosciences; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_216386 Copy
Species: Other
Genetic Insert: TALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI
Vector Backbone Description: Vector Backbone:pYPQ121; Vector Types:Plant Expression, Plastid base editing; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_218172 Copy
Species: Other
Genetic Insert: TALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
Vector Backbone Description: Vector Backbone:pYPQ121; Vector Types:Plant Expression, Plastid base editing; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_218174 Copy
Species: Synthetic
Genetic Insert: [PibpA-gfpASV]
Vector Backbone Description: Backbone Marker:Standard European Vector Architecture; Vector Backbone:pSEVA631; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_221134 Copy
Species: Escherichia coli
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pSC101 rep-ts; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_183241 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:pTF505; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_187872 Copy
Species: Other
Genetic Insert: Transposon Tn5-692
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Tn5 derivative Tn5-692 (in plasmid pRL692; GenBank accession no. AF424805) contains a pMB1 oriV; and bears mutations (Zhou, et al. 1998 J. Mol. Biol. 276:913–925) that increase its rate of transposition ca. 100-fold relative to that of Tn5-1058 (Wolk, et al. 1991. Proc. Natl. Acad. Sci. USA 88:5355–5359), providing large numbers of transposon mutants. The erythromycin-resistance gene came from Horinouchi and Weisblum (J. Bacteriol. 150: 804-814, 1982) and the streptomycin-spectinomycin gene came from the Ω-fragment described by Prentki and Krisch (Gene 29, 303-313, 1984) and was purchased as an EcoRI fragment from Amersham.
NOTE--Because Tn5-692 appears able to transpose repeatedly, the user is cautioned not to delay storing strains whose phenotype is of interest.
Additional references:
Miyagishima S-y, Wolk CP, Osteryoung KW (2005) Identification of cyanobacterial cell division genes by comparative and mutational analyses. Mol Microbiol 56: 126-143
Proper citation: RRID:Addgene_70284 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: MCS: (PT7→)HindIII-SphI-PstI(SbfI)-SalI(HincII)-KpnI-SacI-EcoRI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-XhoI(PspXI)-SrfI-AscI-RsrII-BamHI(←PSP6)
Proper citation: RRID:Addgene_70692 Copy
Species: Arabidopsis thaliana
Genetic Insert: Telomere
Vector Backbone Description: Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene was unable to sequence this plasmid due to the repetitive nature of the telomere repeats. The plasmid was instead verified by restriction digest. The gel image is provided on this page under- Resource Information- Addgene Notes.
Proper citation: RRID:Addgene_65721 Copy
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