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http://www.addgene.org/255795

Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTI site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN086 and pIN200, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.

Proper citation: RRID:Addgene_255795 Copy   

•   Source: Addgene

http://www.addgene.org/255797

Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUC site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN097 and pIN202, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.

Proper citation: RRID:Addgene_255797 Copy   

•   Source: Addgene

http://www.addgene.org/251729

Species:
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pCAH01SpR; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Please visit https://doi.org/10.1101/2025.05.28.656465 for bioRxiv preprint.

Proper citation: RRID:Addgene_251729 Copy   

•   Source: Addgene

http://www.addgene.org/40248

Species: Synechococcus elongatus PCC 7942
Genetic Insert: One Step Cloneing Vector for Overexpression
Vector Backbone Description: Vector Backbone:pAM2990; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: pAM2990 was partially digested with SacI and re-ligatedto remove the antihel fragment. The net result is the insertion of the BglII fragment from pAM2255 into BamHI digested pAM1303 in the orientation that Ptrc goes away from the SacI site in pAM1303 (SacI digestion gives a 1.7 KB band). This plasmid can be used as a one-step cloning vector for overexpression.

Proper citation: RRID:Addgene_40248 Copy   

•   Source: Addgene

http://www.addgene.org/183241

Species: Escherichia coli
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pSC101 rep-ts; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_183241 Copy   

•   Source: Addgene

http://www.addgene.org/187872

Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:pTF505; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_187872 Copy   

•   Source: Addgene

http://www.addgene.org/100917

Species: E. coli
Genetic Insert: RelA fragment from E.coli with point mutation E335Q
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_100917 Copy   

•   Source: Addgene

http://www.addgene.org/101836

Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431E T432E with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_101836 Copy   

•   Source: Addgene

http://www.addgene.org/101846

Species: NA
Genetic Insert: sp/st resistance casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_101846 Copy   

•   Source: Addgene

http://www.addgene.org/101829

Species: S. elongatus PCC7942
Genetic Insert: RpaA with its native promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_101829 Copy   

•   Source: Addgene

http://www.addgene.org/65721

Species: Arabidopsis thaliana
Genetic Insert: Telomere
Vector Backbone Description: Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene was unable to sequence this plasmid due to the repetitive nature of the telomere repeats. The plasmid was instead verified by restriction digest. The gel image is provided on this page under- Resource Information- Addgene Notes.

Proper citation: RRID:Addgene_65721 Copy   

•   Source: Addgene

http://www.addgene.org/70692

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: MCS: (PT7→)HindIII-SphI-PstI(SbfI)-SalI(HincII)-KpnI-SacI-EcoRI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-XhoI(PspXI)-SrfI-AscI-RsrII-BamHI(←PSP6)

Proper citation: RRID:Addgene_70692 Copy   

•   Source: Addgene

http://www.addgene.org/70284

Species: Other
Genetic Insert: Transposon Tn5-692
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Tn5 derivative Tn5-692 (in plasmid pRL692; GenBank accession no. AF424805) contains a pMB1 oriV; and bears mutations (Zhou, et al. 1998 J. Mol. Biol. 276:913–925) that increase its rate of transposition ca. 100-fold relative to that of Tn5-1058 (Wolk, et al. 1991. Proc. Natl. Acad. Sci. USA 88:5355–5359), providing large numbers of transposon mutants. The erythromycin-resistance gene came from Horinouchi and Weisblum (J. Bacteriol. 150: 804-814, 1982) and the streptomycin-spectinomycin gene came from the Ω-fragment described by Prentki and Krisch (Gene 29, 303-313, 1984) and was purchased as an EcoRI fragment from Amersham. NOTE--Because Tn5-692 appears able to transpose repeatedly, the user is cautioned not to delay storing strains whose phenotype is of interest. Additional references: Miyagishima S-y, Wolk CP, Osteryoung KW (2005) Identification of cyanobacterial cell division genes by comparative and mutational analyses. Mol Microbiol 56: 126-143

Proper citation: RRID:Addgene_70284 Copy   

•   Source: Addgene

http://www.addgene.org/160171

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid

Proper citation: RRID:Addgene_160171 Copy   

•   Source: Addgene

http://www.addgene.org/160169

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid

Proper citation: RRID:Addgene_160169 Copy   

•   Source: Addgene

http://www.addgene.org/160261

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid

Proper citation: RRID:Addgene_160261 Copy   

•   Source: Addgene

http://www.addgene.org/160262

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid

Proper citation: RRID:Addgene_160262 Copy   

•   Source: Addgene

http://www.addgene.org/113637

Species: Synthetic
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_113637 Copy   

•   Source: Addgene

http://www.addgene.org/132663

Species: Other
Genetic Insert: aadA
Vector Backbone Description: Backbone Marker:-; Backbone Size:7000; Vector Backbone:pAM5406; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_132663 Copy   

•   Source: Addgene

http://www.addgene.org/128402

Species: C. tetani
Genetic Insert: Tetanus toxin light chain domain
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_128402 Copy   

•   Source: Addgene