Plasmids   Select Another Resource Report Type

http://www.addgene.org/8633

Species: Homo sapiens
Genetic Insert: Human p150,95
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This clone will direct the expression of functional p150,95 upon cotransfection with the beta2 subunit in a variety of cell types. This sequence has been deposited in Genbank with accession number Y00093. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).

Proper citation: RRID:Addgene_8633 Copy   

•   Source: Addgene

http://www.addgene.org/200993

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6393; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Ec100D pir-116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell. This strain is naturally resistant to streptomycin

Proper citation: RRID:Addgene_200993 Copy   

•   Source: Addgene

http://www.addgene.org/182547

Species:
Genetic Insert: H5_Vietnam_1203_04_Y161A
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_182547 Copy   

•   Source: Addgene

http://www.addgene.org/64946

Species:
Genetic Insert: Flp recombinase
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFLP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: GenBank Accession # AY597273 Compared to the Genbank reference sequence AY597273, Addgene's sequencing results found a T350I mutation in the tetracycline cassette and a G5D mutation in FLP recombinase. The effect of these differences is unknown.

Proper citation: RRID:Addgene_64946 Copy   

•   Source: Addgene

http://www.addgene.org/64951

Species:
Genetic Insert: FRT-TcR-FRT
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pFRT2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: GenBank Accession #AY712950 Compared to the Genbank reference sequence AY712950, Addgene's sequencing results found a T350I mutation in the tetracycline cassette. The effect of this difference is unknown.

Proper citation: RRID:Addgene_64951 Copy   

•   Source: Addgene

http://www.addgene.org/67273

Species:
Genetic Insert: tetA
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMJH46; Vector Types:Bacterial Expression, Red Recombinase, temperature sensitive; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Conjugally transferable red recombinase vector pMJH65

Proper citation: RRID:Addgene_67273 Copy   

•   Source: Addgene

http://www.addgene.org/74110

Species:
Genetic Insert: tetA(C)
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-56, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_74110 Copy   

•   Source: Addgene

http://www.addgene.org/74104

Species: Synthetic
Genetic Insert: tetA(C)
Vector Backbone Description: Vector Backbone:pUCBM21; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_74104 Copy   

•   Source: Addgene

http://www.addgene.org/80388

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Synthetic Biology, ; Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_80388 Copy   

•   Source: Addgene

http://www.addgene.org/154035

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Diatom cloning; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: selectable marker added for centric diatoms

Proper citation: RRID:Addgene_154035 Copy   

•   Source: Addgene

http://www.addgene.org/13588

Species: Homo sapiens
Genetic Insert: integrin alpha 9 / alpha 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: See article for cloning details.

Proper citation: RRID:Addgene_13588 Copy   

•   Source: Addgene

http://www.addgene.org/13585

Species: Homo sapiens
Genetic Insert: integrin alpha 9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Please note that the first 41 amino acids of the integrin alpha insert show greater identity to integrin alpha-5 rather than integrin alpha-9

Proper citation: RRID:Addgene_13585 Copy   

•   Source: Addgene

http://www.addgene.org/13582

Species: Homo sapiens
Genetic Insert: integrin beta 6 777T
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_13582 Copy   

•   Source: Addgene

http://www.addgene.org/157933

Species: Synthetic
Genetic Insert: A/PuertoRico/8/34 hemagglutinin
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_157933 Copy   

•   Source: Addgene

http://www.addgene.org/25867

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_25867 Copy   

•   Source: Addgene

http://www.addgene.org/32697

Species: Homo sapiens
Genetic Insert: RAP1
Vector Backbone Description: Backbone Marker:Anumanthan et al. 1998; Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_32697 Copy   

•   Source: Addgene

http://www.addgene.org/42600

Species: Caenorhabditis elegans
Genetic Insert: let-363 cDNA
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.

Proper citation: RRID:Addgene_42600 Copy   

•   Source: Addgene

http://www.addgene.org/42601

Species: Caenorhabditis elegans
Genetic Insert: daf-15
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain. Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.

Proper citation: RRID:Addgene_42601 Copy   

•   Source: Addgene

http://www.addgene.org/21900

Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Addgene sequencing results show that only half of the leurin zipper is deleted, protein ends at amino acid 133. Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.

Proper citation: RRID:Addgene_21900 Copy   

•   Source: Addgene

http://www.addgene.org/17841

Species: Mus musculus
Genetic Insert: GlyCAM-1/IgG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:

Proper citation: RRID:Addgene_17841 Copy   

•   Source: Addgene