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Vector Backbone Description: Backbone Marker:Partially Addgene; Backbone Size:4904; Vector Backbone:Constructed from MoClo Yeast Toolkit (Kit # 1000000061) and own yeast homology arms (Addgene plasmids 125030 and 125063); Vector Types:Yeast Expression, Kluyveromyces marxianus; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33176787
Proper citation: RRID:Addgene_160142 Copy
Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160144 Copy
Species: Homo sapiens
Genetic Insert: FLIP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15367674
Comments: A 1,460-bp sequence containing the promoter region of the FLIP gene was amplified using the BAC clone RP11-536118 (Children’s Hospital Oakland Research Institute, Oakland, Calif.) and inserted into the luciferase-expressing reporter plasmid pGL3-basic (Promega). The sequence corresponds to positions –1179 to +281 relative to the proposed transcriptional start site of CFLAR (FLIP) exon 1 (accession no. AB038965).
Proper citation: RRID:Addgene_16016 Copy
Species: Homo sapiens
Genetic Insert: CARP1
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15069192
Proper citation: RRID:Addgene_16014 Copy
Species: Homo sapiens
Genetic Insert: CARP2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15069192
Proper citation: RRID:Addgene_16013 Copy
Species: Homo sapiens
Genetic Insert: KILLER/DR5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777207
Comments: A 3.6 Kb Pst1 fragment from the KILLER/DR5 P1 clone which includes 1.6 Kb upstream of the ATG through Intron 2 in KILLER/DR5 genomic DNA.
Proper citation: RRID:Addgene_16012 Copy
Species: Homo sapiens
Genetic Insert: cmyc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15367674
Comments: Contains cmyc with Kozak.
Proper citation: RRID:Addgene_16011 Copy
Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160147 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160172 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160174 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160175 Copy
Vector Backbone Description: Backbone Marker:Partially Addgene; Backbone Size:5640; Vector Backbone:Constructed from MoClo Yeast Toolkit (Kit # 1000000061) and own yeast homology arms (Addgene plasmids 125032 and 125065); Vector Types:Yeast Expression, Kluyveromyces marxianus; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33176787
Proper citation: RRID:Addgene_160176 Copy
Species: Homo sapiens
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: Targets collybistin MTL-CBSH3- mRNA (target is located in the protein coding region. Putative rat sequence based on human sequence - targets nucleotides 813-834 of human NM_001353924.2. Targets MTL collybistin isoforms without SH3 domain). There is also the mutated shRNA control for this isoform (Addgene #160211).
Proper citation: RRID:Addgene_160210 Copy
Vector Backbone Description: Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160178 Copy
Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Addgene Plasmid #160210).
Proper citation: RRID:Addgene_160211 Copy
Vector Backbone Description: Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160179 Copy
Vector Backbone Description: Backbone Marker:Partially Addgene; Backbone Size:5129; Vector Backbone:Constructed from MoClo Yeast Toolkit (Kit # 1000000061) and own yeast homology arms (Addgene plasmids 125033 and 125066); Vector Types:Yeast Expression, Kluyveromyces marxianus; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33176787
Proper citation: RRID:Addgene_160215 Copy
Vector Backbone Description: Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32955754
Comments: Clone gene of interest using Tag_Fwd and Tag_Rev primers from the InteBac suite
Proper citation: RRID:Addgene_160162 Copy
Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Addgene Plasmid # 160208).
Proper citation: RRID:Addgene_160209 Copy
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32955754
Proper citation: RRID:Addgene_160168 Copy
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