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Species: Caenorhabditis elegans
Genetic Insert: pEXP[Pmlc-2 | histamine | rpl-3 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33437931
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159797 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159798 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Prpl-3 | histamine | rpl-3 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33437931
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159796 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting HOXD13 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32351707
Proper citation: RRID:Addgene_159793 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159794 Copy
Species: Homo sapiens
Genetic Insert: spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32351707
Comments: Other insert name: RT&PBS of pegRNA_HOXD13_CtoG targting HOXD13 gene
Proper citation: RRID:Addgene_159791 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting VEGFA gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32351707
Proper citation: RRID:Addgene_159790 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (only amino acids 1-316)(includes MTBD and P123 domains)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159728 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (full length, 1-963 amino acids), K963
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159727 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159848 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159843 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159844 Copy
Vector Backbone Description: Backbone Size:9875; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.60, Ligation number L3808.
Proper citation: RRID:Addgene_1598 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159854 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159855 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159852 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159851 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159869 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modified from the pHEE401E T-DNA vector described by Wang &al. (2015) Genome Biol 16: 144, doi:10.1186/s13059-015-0715-0: the Hygromycin selection maker of the T-DNA was exchanged with a cassette enabling selection on the basis of CFP-fluorescence in seeds; the egg-cell specific promoter of Cas9 was exchanged with a ubiquitin promoter. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253203v1 for BioRxiv preprint
Proper citation: RRID:Addgene_159747 Copy
Species: Homo sapiens
Genetic Insert: DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human DMD in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorescence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for BioRxiv preprint
Proper citation: RRID:Addgene_159744 Copy
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