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Genetic Insert: OVA-SIY
Vector Backbone Description: Backbone Marker:Available at Addgene (#20905); Backbone Size:0; Vector Backbone:Luc.Cre (aka: UbC.Luciferase.PGK.Cre); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22777 Copy
Species: Homo sapiens
Genetic Insert: estrogen receptor beta
Vector Backbone Description: Backbone Size:7136; Vector Backbone:pCDNA3.1/nv5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17909066
Proper citation: RRID:Addgene_22770 Copy
Species: Mus musculus
Genetic Insert: Myelin Transcription Factor 1
Vector Backbone Description: Backbone Marker:R. Armstrong; Backbone Size:5635; Vector Backbone:pIRESRed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14962745
Comments: Mu Myt1 complete sequence includes 7 Zn Fingers and a Myc Tag. This was subcloned into pIRES/Red made by R. A. Armstrong. The DsRed does not express due to problem with IRES
This Myt1-7zf DNA was cut from the full-length clone (pMycMyt1-7zf) which was a gift of Dr. Jacob Hecksher-Sorensen.
Proper citation: RRID:Addgene_22652 Copy
Species: Homo sapiens
Genetic Insert: Rev Erb alpha
Vector Backbone Description: Backbone Marker:Available at Addgene (#16405); Backbone Size:9000; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20018698
Proper citation: RRID:Addgene_22745 Copy
Species: Mus musculus
Genetic Insert: shRev-Erbalpha
Vector Backbone Description: Backbone Marker:Available at Addgene (#8453); Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20018698
Comments: targeting sequence: GCGCTTTGCATCGTTGTTCAA
Proper citation: RRID:Addgene_22747 Copy
Species: Mus musculus
Genetic Insert: shALAS-1
Vector Backbone Description: Backbone Marker:Available at Addgene (#16404); Backbone Size:8300; Vector Backbone:pAdTrack; Vector Types:Mammalian Expression, Adenoviral, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20018698
Comments: targeting sequence: CCAAGATAGTAGCATTTGAAA
Proper citation: RRID:Addgene_22748 Copy
Genetic Insert: microRNA 9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5699; Vector Backbone:pcDNA-6.2 GW/EmGFP miR-neg control; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:18987208
Proper citation: RRID:Addgene_22741 Copy
Genetic Insert: modified pCMV-DsRed-Express (Clontech)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4600; Vector Backbone:pCMV-DsRed-Express; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987208
Comments: modified pCMV-DsRed-Express (Clontech)) Have inserted 2 sites (NheI and BamHI upstream of DsRed-Express cassette and 2 sites downstream (BglII and NheI) = NheI-BamHI-DsRed-Express-BglII-EcoRI
Proper citation: RRID:Addgene_22743 Copy
Genetic Insert: CXCR4 control sponge
Vector Backbone Description: Backbone Marker:Weinberg lab (Addgene#:1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19524507
Comments: Subclond from CMV-CXCR4 control sponge, as described in Ebert et al. (Nature Methods, 2007)
Sequence of sponge: AAGUUUUCAGAAAGCUAACA
Proper citation: RRID:Addgene_22744 Copy
Species: Mus musculus
Genetic Insert: Myelin Transcription Factor 1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pCR-Blunt II TOPO; Vector Types:PCR cloning vector; Bacterial Resistance:Kanamycin
Comments: 580bp Myt1 insert can be released with EcoRI. This is the 580bp cDNA probe for Northern blots. Paper is in preparation. Tentatively titled "The Myelin Transcription Factor 1 (Myt1) is Essential for Neural Development"
Proper citation: RRID:Addgene_22740 Copy
Species: Mus musculus
Genetic Insert: PPARalpha
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4000; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The PPARa cDNA was isolated from an adipocyte lambda ZAPII cDNA library (Tontonoz et al 1994 Genes Dev 8:1224-1234).
Proper citation: RRID:Addgene_22751 Copy
Species: Mus musculus
Genetic Insert: Trp53
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19668191
Comments: additional article reference: Shinmura, K. et al. Direct evidence for the role of centrosomally localized p53 in the regulation of centrosome duplication. Oncogene. 2007 May 3;26(20):2939-44. Epub 2006 Oct 30.
Proper citation: RRID:Addgene_22727 Copy
Species: Mus musculus
Genetic Insert: Trp53
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19668191
Comments: additional article reference: Bowman, T. et al. Tissue-specific inactivation of p53 tumor suppression in the mouse. Genes Dev. 1996 Apr 1;10(7):826-35.
Proper citation: RRID:Addgene_22729 Copy
Species: mushroom
Genetic Insert: a variant of Discosoma sp. red fluorescent protein
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo; Backbone Size:4800; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19668191
Proper citation: RRID:Addgene_22724 Copy
Species: Mus musculus
Genetic Insert: Trp53
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19668191
Proper citation: RRID:Addgene_22725 Copy
Species: Homo sapiens
Genetic Insert: E1A-like inhibitor of differentiation 1
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4938; Vector Backbone:pGex2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11073989
Proper citation: RRID:Addgene_22689 Copy
Genetic Insert: Lox71-FRT-PGK-hygro-FRT-Lox2272
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3060; Vector Backbone:pBluescript KS+; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21324933
Comments: Please note that the plasmid names and lox sites have been corrected since publication.
Proper citation: RRID:Addgene_22731 Copy
Genetic Insert: miR30
Vector Backbone Description: Backbone Size:7500; Vector Backbone:MSCV2.2 with WPRE cloned into ClaI site is the base vector; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Comments: See FLIP vector protocols (PDF from this page) for more information.
This vector contains the SwaI site for cloning multiple shRNAs into a single vector.
Proper citation: RRID:Addgene_22699 Copy
Genetic Insert: Lox71-MCS-Lox2272
Vector Backbone Description: Backbone Size:2905; Vector Backbone:pBluescript KS+; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21324933
Comments: Please note that the plasmid names and lox sites have been corrected since publication.
Proper citation: RRID:Addgene_22732 Copy
Genetic Insert: Lox66-pU(delta)TK-EM7Neo-Lox2272
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2969; Vector Backbone:pBluescript KS+; Vector Types:Cre/Lox, BAC recombineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21324933
Comments: Please note that the plasmid names and lox sites have been corrected since publication.
Proper citation: RRID:Addgene_22733 Copy
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