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Species: Synthetic
Genetic Insert: 30x 147-bp Widom 601
Vector Backbone Description: Backbone Marker:Erik Jorgensen (Addgene plasmid #73738); Backbone Size:2438; Vector Backbone:pMLS280; Vector Types:DNA amplification; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40291647
Comments: Cloning carried out as in: Dyer PN, Edayathumangalam RS, White CL, Bao Y, Chakravarthy S, Muthurajan UM, Luger K. Reconstitution of nucleosome core particles from recombinant histones and DNA. Methods Enzymol. 2004;375:23-44. doi: 10.1016/s0076-6879(03)75002-2. PMID: 14870657.
DNA purification as in: Paintsil EA, Morrison EA. Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles. Methods Mol Biol. 2023;2599:163-175. doi: 10.1007/978-1-0716-2847-8_12. PMID: 36427149.
Nucleosome core particle reconstitutions largely as in: Hammonds EF, Morrison EA. Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. Methods Mol Biol. 2023;2599:177-190. doi: 10.1007/978-1-0716-2847-8_13. PMID: 36427150. Exception: Suggest reconstitution molar ratios of 1:1:2 DNA:H3/H4-tetramer:H2A/H2B-dimer.
Please visit https://doi.org/10.1101/2025.04.09.647968 for bioRxiv preprint.
Proper citation: RRID:Addgene_240634 Copy
Species: Synthetic
Genetic Insert: VP16
Vector Backbone Description: Backbone Size:6774; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40500178
Proper citation: RRID:Addgene_241843 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Vector Backbone:pFC223C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_236870 Copy
Species: Lactiplantibacillus plantarum
Genetic Insert: Lactate racemase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39679682
Proper citation: RRID:Addgene_232792 Copy
Species: Homo sapiens
Genetic Insert: Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2
Vector Backbone Description: Backbone Marker:Lamminmäki Group, Department of Life Technologies, University of Turku; Backbone Size:6000; Vector Backbone:pLK06Hpp; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40885348
Proper citation: RRID:Addgene_239861 Copy
Species: Synthetic
Genetic Insert: H2B-iRFP
Vector Backbone Description: Vector Backbone:pminiTol2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40906841
Proper citation: RRID:Addgene_240802 Copy
Species: SARS-CoV-2
Genetic Insert: GFP-Nsp3(1-111)
Vector Backbone Description: Backbone Size:5251; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39464032
Proper citation: RRID:Addgene_242947 Copy
Species: Synthetic
Genetic Insert: CIBN-eGFP
Vector Backbone Description: Vector Backbone:pminiTol2; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40906841
Proper citation: RRID:Addgene_240804 Copy
Species: inflluenza A virus
Genetic Insert: mvRNA NP71.2
Vector Backbone Description: Vector Backbone:pPol I; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_240808 Copy
Species: Lactiplantibacillus plantarum
Genetic Insert: Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39679682
Proper citation: RRID:Addgene_232793 Copy
Species: Homo sapiens
Genetic Insert: Anti-FLAG frankenbody
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40366872
Proper citation: RRID:Addgene_241140 Copy
Species: Synthetic
Genetic Insert: Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
Vector Backbone Description: Backbone Size:12868; Vector Backbone:pBI121; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15020753
Comments: See the Supplemental Document under Resource Information for more details, including annotated maps. Figure 1 of the Gao et al. (2004) paper also gives an overview of the gene cassettes.
Proper citation: RRID:Addgene_240451 Copy
Species: Synthetic
Genetic Insert: Fusion of soluble modified ratiometric pHluorin and Aequorin
Vector Backbone Description: Backbone Size:12868; Vector Backbone:pBI121; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15020753
Comments: See the Supplemental Document under Resource Information for more details, including annotated maps. Figure 1 of the Gao et al. (2004) paper also gives an overview of the gene cassettes.
Proper citation: RRID:Addgene_240453 Copy
Species: Aequorea victoria; Thermus aquaticus, Discosoma sp.
Genetic Insert: cyt-NAPstar2
Vector Backbone Description: Backbone Size:9954; Vector Backbone:pSS02; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39702652
Proper citation: RRID:Addgene_241272 Copy
Species: Synthetic
Genetic Insert: eLACCO2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41162396
Proper citation: RRID:Addgene_223348 Copy
Genetic Insert: ChroME2f_GCaMP8m_ST
Vector Backbone Description: Vector Backbone:pAAV-hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_227783 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1_mandi
Vector Backbone Description: Vector Backbone:pBD-GAL4-cam; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25652827
Comments: Cutler lab uses this with yeast strain Mav99 but has tested it with Mav203 (commercially available) and it works well with that strain too. Please visit https://doi.org/10.1101/2025.05.15.654352 for bioRxiv preprint.
Proper citation: RRID:Addgene_241279 Copy
Species: Synthetic
Genetic Insert: deLACCO1
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41162396
Proper citation: RRID:Addgene_223344 Copy
Species: Synthetic
Genetic Insert: eLACCO2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41162396
Proper citation: RRID:Addgene_223345 Copy
Genetic Insert: Cas9-gRNA
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric\_BB-Cb-hSpCas9; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40950402
Proper citation: RRID:Addgene_241839 Copy
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