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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
cLS-F2
 
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RRID:Addgene_32617 rGH-PAL Gallus gallus Ampicillin PMID:21060789 Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 0
pLKO.DEST.YFP
 
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RRID:Addgene_32683 Chloramphenicol and Ampicillin PMID:21418658 The full plasmid sequence was assembled and may contain minor discrepancies that should not effect the function of the plasmid. Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-19 02:17:10 0
pCMV myc DAF-12(P687S)
 
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RRID:Addgene_32567 DAF-12(P687S) Caenorhabditis elegans Ampicillin PMID:21814518 Vector Backbone:pCMV myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Proline 687 to Serine 2026-09-19 02:17:09 0
pCAG:mRFP1
 
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1+ mentions
RRID:Addgene_32600 mRFP1 Ampicillin PMID:15996270 The mRFP1 coding sequence was amplified by PCR using primers 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg) and 3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc) using Pfx Polymerase (Invitrogen). The resulting product was cloned into the EcoRI site of pCAGGS to generate pCXmRFP1. Transient transfection of Cos-7 cells using Fugene 6 Transfection Reagent as per manufacturer's recommendations (Roche) was used to evaluate pCX-mRFP1, and verify that it produced robust red fluorescence More Information: https://www.ncbi.nlm.nih.gov/pubmed/12060735 A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12) Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 4
pENTR.hU6hH1
 
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RRID:Addgene_32686 Kanamycin PMID:21418658 Backbone Marker:Invitrogen; Backbone Size:2720; Vector Backbone:pENTR4; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin 2026-09-19 02:17:10 0
pCAG:GPI-GFP
 
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10+ mentions
RRID:Addgene_32601 GPI-GFP Ampicillin PMID:16604528 Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 13
pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65
 
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RRID:Addgene_32569 EGFP Ampicillin PMID:19212426 This plasmid grows quite slowly. Please allow up to two days for growth. Backbone Size:7469; Vector Backbone:pTYF; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 0
pCAG:myr-Venus
 
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1+ mentions
RRID:Addgene_32602 myr-Venus Ampicillin PMID:16604528 To generate myristoylated fluorescent fusion proteins, an N-terminal myristoylation tag derived from Src was generated by adding the sequences MGSSKSKPK to the N-terminus of any given fluorescent protein. This was achieved by using the following oligonucleotide: 5′ GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GTG AGC AAG GGC GAG GAG CTG). The GFP and Venus coding sequences were amplified from pEGFP-N1 (BD Biosciences, San Jose, CA) and pCS2-Venus (Nagai et al., 2002) to generate myr-GFP and myr-Venus, respectively, by high-fidelity polymerase chain reaction (PCR) using Pfx Polymerase (Invitrogen, La Jolla, CA) with the 5′ myristoylation primer combined with a 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG TCC) primer, respectively. The resulting product was cloned into the EcoRI site of pCAGGS to generate pCX::myr-EGFP and pCX::myr-Venus Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 5
pISce1-D2EGFP
 
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RRID:Addgene_32674 Ampicillin Backbone Size:3500; Vector Backbone:pISce1; Vector Types:Zebrafish Transgenesis; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 0
pENTR-L5-Kir2.1-mCherry-L2
 
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1+ mentions
RRID:Addgene_32669 Kir2.1-mCherry Mus musculus Kanamycin PMID:21772812 Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-09-19 02:17:10 4
pSADdeltaG-FLPo-DsRedX
 
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1+ mentions
RRID:Addgene_32650 FLPo Ampicillin PMID:21867879 Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 1
pGEMTEZ-Kir2.1
 
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1+ mentions
RRID:Addgene_32641 Kir2.1 Mus musculus Ampicillin PMID:15157418 Addgene's sequencing results identified a single nucleotide insertion at bp#60/61 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results. Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 3
pTetO-Kir2.1-IRES-TLZ
 
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1+ mentions
RRID:Addgene_32642 tetO-Kir2.1-IRES-TLZ Mus musculus Ampicillin PMID:15157418 Addgene's sequencing results identified a single nucleotide a single nucleotide deletion at bp#1135 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results. Backbone Marker:Stratagene; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 1
pSADdeltaG-GFP-rtTA
 
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RRID:Addgene_32648 GFP Ampicillin PMID:21867879 Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 0
pSADdeltaG-ChR2-mCherry
 
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1+ mentions
RRID:Addgene_32646 ChR2 Ampicillin PMID:21867879 Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 1
pSADdeltaG-GFP-ER(T2)CreER(T2)
 
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1+ mentions
RRID:Addgene_32649 GFP Ampicillin PMID:21867879 Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 1
pENTR-R4r-ECFP-R3r
 
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RRID:Addgene_32594 ECFP Kanamycin PMID:21772812 Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-09-19 02:17:09 0
pCAG:H2B-EGFP
 
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1+ mentions
RRID:Addgene_32599 H2B-EGFP Ampicillin PMID:15619330 Please note that the plasmid contains a duplication of the H2B-EGFP region as compared to the original depositor sequence. The depositor has confirmed that the plasmid functions as described in the associated publication. The coding sequence for the human histone H2B gene (X57127) was amplified from genomic DNA by PCR using Pfx Polymerase (Invitrogen). The resulting product was cloned into pCR4 TOPO (Invitrogen) to generate pH2B. The H2B fragment was then cloned into plasmids pEGFP-N1, pDsRed2-N1 pDsRedExpress-N1 (BD Biosciences, Inc) in order to generate plasmids pH2B-EGFP, pH2B-DsRed2 and pH2B-DsRedExpress (oligonucleotide sequences are available upon request). The resulting fusions were then re-amplified by PCR and cloned into the XhoI site of pCAGGS [19] to generate pCX-H2B-EGFP, pCX-H2B-DsRed2 and pCX-H2B-DsRedExpress. Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 2
pCAG:ECFP
 
Resource Report
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1+ mentions
RRID:Addgene_32597 ECFP Ampicillin PMID:12079497 Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:09 1
pcDNA-SADB19P
 
Resource Report
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10+ mentions
RRID:Addgene_32631 SADB19P Rabies virus Ampicillin PMID:21867879 Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-19 02:17:10 15

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