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Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724390.1
Proper citation: RRID:Addgene_145741 Copy
Species: Bos taurus
Genetic Insert: PIK3CA E545K
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16322248
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
Proper citation: RRID:Addgene_14571 Copy
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Jeff Engelman; Backbone Size:0; Vector Backbone:JP1520; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16322248
Comments: Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA). The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).
Proper citation: RRID:Addgene_14570 Copy
Species: Other
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725297.1
Proper citation: RRID:Addgene_145747 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725301.1
Proper citation: RRID:Addgene_145756 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725302.1
Proper citation: RRID:Addgene_145757 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725307.1
Proper citation: RRID:Addgene_145754 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 3'-to-5' exonuclease
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725309.1
Proper citation: RRID:Addgene_145755 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724390.1
Proper citation: RRID:Addgene_145753 Copy
Species: Other
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724392.1
Proper citation: RRID:Addgene_145751 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725304.1
Proper citation: RRID:Addgene_145758 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725297.1
Proper citation: RRID:Addgene_145759 Copy
Species: Homo sapiens
Genetic Insert: kappa-myc-dK-2xG4S-TMst
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5358; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786268
Comments: Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
http://pathways.mbic.cmu.edu/
For more information about this plasmid, please see the associated publication (PMID: 32786268).
Proper citation: RRID:Addgene_145766 Copy
Species: Other
Genetic Insert: SARS-CoV-2 ORF7a protein
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724395.1
Proper citation: RRID:Addgene_145701 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725305.1
Proper citation: RRID:Addgene_145702 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725306.1
Proper citation: RRID:Addgene_145700 Copy
Species: HIV-1
Genetic Insert: Capsid
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:2863; Vector Backbone:pTZ19U; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33033190
Proper citation: RRID:Addgene_145785 Copy
Species: HIV-1
Genetic Insert: Capsid
Vector Backbone Description: Backbone Marker:Bio-Rad; Backbone Size:2863; Vector Backbone:pTZ19U; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33033190
Proper citation: RRID:Addgene_145784 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009725302.1
Proper citation: RRID:Addgene_145709 Copy
Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:1462; Vector Backbone:ori-013 - marker | cmR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: NCBI Reference Sequence: YP_009724390.1
Proper citation: RRID:Addgene_145706 Copy
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