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Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34958 Copy
Genetic Insert: PSmOrange2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22900938
Comments: https://www.ncbi.nlm.nih.gov/nuccore/JQ392581
Proper citation: RRID:Addgene_34957 Copy
Species: Strongylocentrotus purpuratus
Genetic Insert: ABCB1a
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34956 Copy
Species: Strongylocentrotus purpuratus
Genetic Insert: ABCB4a
Vector Backbone Description: Backbone Marker:Gokirmak et al., 2012; Backbone Size:4851; Vector Backbone:pCS2+8NmCherry; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34941 Copy
Species: Strongylocentrotus purpuratus
Genetic Insert: ABCG2a
Vector Backbone Description: Backbone Marker:Gokirmak et al., 2012; Backbone Size:4860; Vector Backbone:pCS2+8CmCherry; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34946 Copy
Species: Strongylocentrotus purpuratus
Genetic Insert: ABCB1a
Vector Backbone Description: Backbone Marker:Gokirmak et al., 2012; Backbone Size:4860; Vector Backbone:pCS2+8NCerulean; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34944 Copy
Species: Homo sapiens
Genetic Insert: ADRB2
Vector Backbone Description: Backbone Marker:Gateway; Backbone Size:8100; Vector Backbone:MSCV-IP N-HA only; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_34895 Copy
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34931 Copy
Species: Homo sapiens
Genetic Insert: TBPL1
Vector Backbone Description: Backbone Marker:Gateway; Backbone Size:8100; Vector Backbone:MSCV-IP N-HA only; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_34896 Copy
Species: Homo sapiens
Genetic Insert: p110β
Vector Backbone Description: Backbone Marker:Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5; Backbone Size:2949; Vector Backbone:pBSFI; Vector Types:Adaptor plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432180
Proper citation: RRID:Addgene_34891 Copy
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34935 Copy
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34938 Copy
Vector Backbone Description: Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_34937 Copy
Genetic Insert: CAG-Floxed Synaptophysin-tdTomato
Vector Backbone Description: Backbone Size:13898; Vector Backbone:Rosa26 Targeting Vector; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22446880
Proper citation: RRID:Addgene_34881 Copy
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescriptII; Vector Types:Moss Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18803774
Comments: Scientists using this plasmid in a publication should acknowledge Drs. Tomoaki Nishiyama and Mitsuyasu Hasebe in the publication.
Proper citation: RRID:Addgene_34888 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Neomycin resistance vector for drug selection in worms.
This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34920 Copy
Vector Backbone Description: Backbone Size:4453; Vector Backbone:unknown; Vector Types:Mammalian Expression, Mouse Targeting, tet inducible; Bacterial Resistance:Ampicillin
Comments: Targeting construct for generating tet inducible mouse lines.
Clone into EcoRV.
Cut with NotI and Sfi for oocyte injection fragment.
Proper citation: RRID:Addgene_34925 Copy
Genetic Insert: Lck-GCaMP5G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3950; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23035093
Comments: Lck-GCaMP5G is a fusion between the Lck domain (https://www.ncbi.nlm.nih.gov/pubmed/21205365) and cytosolic GCaMP5G (http://www.addgene.org/31788). This construct targets GCaMP5G to the plasma membrane.
Proper citation: RRID:Addgene_34924 Copy
Species: Homo sapiens
Genetic Insert: p53 DNA-binding (Res 94-358, deletion 293-321)
Vector Backbone Description: Backbone Size:5194; Vector Backbone:pT5T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21522129
Proper citation: RRID:Addgene_34923 Copy
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy.
Proper citation: RRID:Addgene_34929 Copy
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