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Species: Homo sapiens
Genetic Insert: OTUD5
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:0; Vector Backbone:pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22610 Copy
Species: Homo sapiens
Genetic Insert: USP36
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22579 Copy
Species: Homo sapiens
Genetic Insert: USP18
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22572 Copy
Species: Homo sapiens
Genetic Insert: USP20
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22573 Copy
Species: Homo sapiens
Genetic Insert: USP21
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22574 Copy
Species: Homo sapiens
Genetic Insert: USP15
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22570 Copy
Species: Homo sapiens
Genetic Insert: JOSD1
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22547 Copy
Species: Homo sapiens
Genetic Insert: EIF3S3(1-348aa)
Vector Backbone Description: Backbone Marker:Harper_Lab; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22545 Copy
Species: Homo sapiens
Genetic Insert: COPS5
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22541 Copy
Species: Homo sapiens
Genetic Insert: PARP11
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22556 Copy
Species: Homo sapiens
Genetic Insert: OTUB1
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:MSCV-N-Flag-HA-IRES-PURO; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22551 Copy
Species: Homo sapiens
Genetic Insert: OTUB2
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19615732
Proper citation: RRID:Addgene_22552 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Proper citation: RRID:Addgene_22763 Copy
Species: Drosophila melanogaster
Genetic Insert: micro-RNA 315
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Proper citation: RRID:Addgene_22765 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16177138
Proper citation: RRID:Addgene_22760 Copy
Species: Drosophila melanogaster
Genetic Insert: micro RNA 13b-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5348; Vector Backbone:pAc5.1A; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Proper citation: RRID:Addgene_22762 Copy
Species: Mus musculus
Genetic Insert: Myelin Transcription Factor 1
Vector Backbone Description: Backbone Marker:gift from Dr. Randy Thresher; Backbone Size:8272; Vector Backbone:pOSfrtloxP; Vector Types:Targeting vector; Bacterial Resistance:Ampicillin
Comments: 4899bp of genomic Myt1 sequences with MET of Exon 1 as 5' region of homolgy (NotI/PmeI). Inserted upstream of eGFP/icre/frt/loxP. 1403bp of genomic Myt1 sequence starts at exon 2 and extends past Exon 3 as 3' region of homology (ClaI/NheI) Inserted downstream of eGFP/icre/frt/loxP.
The iCre-PolyA cassette was PCR'd out of the pBlue.iCre (a gift of Dr. Rolf Sprengel) with HindIII/KpnI ends. The iCre cassette was ligated to peGFP-C2 (HindIII/KpnI) (Clontech). The eGFP-iCre-PolyA cassette was PCR'd out with SalI ends. This eGFP-icre-PolyA (SalI) cassette was then cloned into pOSfrtloxP (Dr. Randy Thresher) upstream of the frt and loxP sites. 4886bp of 5' Mu genomic Myt1 DNA was inserted upstream of eGFP and the 1404 bp 3' Mu Genomic DNA was inserted downstream NEO gene. The entire pMyt1GFPicre-Knock-In has been sequenced and verified.
Proper citation: RRID:Addgene_22657 Copy
Species: A. victoria
Genetic Insert: CyPet
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCyPet-C1-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19693013
Proper citation: RRID:Addgene_22779 Copy
Species: Mus musculus
Genetic Insert: mSin3B
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5565; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15935060
Comments: Used to see if truncated form of mSin3b would be biologically active when binding Mouse Myt1
This short form of mSIN3B was PCR'd from the full-length clone (pcDNAAmSin3B-Flag) which was a gift of Dr. Ronald dePinho.
Proper citation: RRID:Addgene_22653 Copy
Genetic Insert: fret-STOP-fret
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11751630
Comments: Plasmid containing FRT-STOP-FRT cassette for use in expressing genes only after Flp-mediated recombination.
It's like the LSL cassette ( http://www.addgene.org/11584 but this one is a FSF cassette
Proper citation: RRID:Addgene_22774 Copy
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