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Species: Mus musculus
Genetic Insert: MKK7a1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14538 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5D B56 delta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Please note that the Addgene verified sequence finds an R210H mutation in PPP2R5D. Other differences compared to the depositor sequence do not affect plasmid function.
Proper citation: RRID:Addgene_14536 Copy
Species: Homo sapiens
Genetic Insert: RUNX2(+10A)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145268 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5B B56 beta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Addgene NGS identified an I346V mutation in the B56β ORF, this is not predicted to impact plasmid function.
Proper citation: RRID:Addgene_14533 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 N (nucleocapsid)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724397.2. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145389 Copy
Species: Homo sapiens
Genetic Insert: TBP(38Q)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145269 Copy
Species: Homo sapiens
Genetic Insert: HOXA13(+7A)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145266 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 2'-O-ribose methyltransferase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725311.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145387 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5A B56 alpha regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
Proper citation: RRID:Addgene_14532 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145384 Copy
Species: Homo sapiens
Genetic Insert: HOXD13wt-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145260 Copy
Species: Homo sapiens
Genetic Insert: HOXD13(+7A)-IDR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145382 Copy
Species: Mus musculus
Genetic Insert: MKK7a1(Ala)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14539 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724394.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725301.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145434 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145431 Copy
Vector Backbone Description: Backbone Size:8224; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD69.39, Ligation number NONE.
Proper citation: RRID:Addgene_1454 Copy
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