Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Xanthamonas oryzae
Genetic Insert: TALE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2676; Vector Backbone:pCR8-GW modified; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21493687
Comments: Gateway compatible vector for assembly of full-length TAL effector genes by Golden Gate cloning. Contains both SD and Kozak sequences immediately upstream of the initial ATG. Designed for TAL DNA-binding domains alone
Proper citation: RRID:Addgene_31031 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_31152 Copy
Species: Xanthamonas oryzae
Genetic Insert: TALEN-FokI nuclease backbone
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pDW1789; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21493687
Comments: Expression of the TAL-FokI homodimer in yeast.
Proper citation: RRID:Addgene_31034 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_31154 Copy
Species: Rattus norvegicus
Genetic Insert: Regulator of G Protein Signaling 4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294233
Proper citation: RRID:Addgene_31157 Copy
Species: Xanthamonas oryzae
Genetic Insert: TALEN-FokI nuclease backbone
Vector Backbone Description: Backbone Size:6519; Vector Backbone:pDW1789_Leu; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21493687
Comments: Expression of the TAL-FokI homodimer in yeast.
NOTE: The GenBank file associated with this plasmid (accessible from the Golden Gate TALEN kit page) erroneously lists this plasmid as having a D450A codon (A--C) FokI mutation.
Proper citation: RRID:Addgene_31035 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK3
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31038 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK2
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31037 Copy
Genetic Insert: basal adenovirus E1b TATA element
Vector Backbone Description: Backbone Size:2810; Vector Backbone:p5E; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: "p5E" refers to 5' entry clones flanked by L4-R1
Proper citation: RRID:Addgene_31158 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31030 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_31151 Copy
Species: Homo sapiens
Genetic Insert: ADAM7
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618342
Proper citation: RRID:Addgene_31141 Copy
Species: Homo sapiens
Genetic Insert: ADAM29
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:6600; Vector Backbone:pCDF1-EF1-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618342
Proper citation: RRID:Addgene_31145 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31027 Copy
Species: firefly luciferase from pGL3-Basic
Genetic Insert: firefly luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty, to measure tetracycline regulation by Tet repressor
Proper citation: RRID:Addgene_31098 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11376165
Proper citation: RRID:Addgene_31130 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19835622
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -218.
Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).
Proper citation: RRID:Addgene_31090 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19835622
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -138.
Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).
Proper citation: RRID:Addgene_31091 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19835622
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in P2 promoter of HNF4A, shield inducibility by destabilizing domain FKBP L106P
Proper citation: RRID:Addgene_31095 Copy
Species: Mus musculus
Genetic Insert: Sfrp2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096311
Comments: For Riboprobe, cut with XbaI. Runoffs with T7 polymerase.
This construct was used in Assimacopoulos et al, J Neurosci. 2003 (PMID:
12878679).
Proper citation: RRID:Addgene_31249 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.