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Genetic Insert: ndmB
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/2C3Oh1ew . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113647 Copy
Genetic Insert: ndmA
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31540989
Comments: https://benchling.com/s/4vRm4arA . Please visit https://www.biorxiv.org/content/early/2018/09/16/419283 for bioRxiv preprint.
Proper citation: RRID:Addgene_113646 Copy
Species: Synthetic
Genetic Insert: IodoYRS and tRNA gene
Vector Backbone Description: Vector Backbone:pACYC184. p15A; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27648665
Comments: https://benchling.com/s/vJhpn35J
Proper citation: RRID:Addgene_113645 Copy
Genetic Insert: sgRNA cloning site
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:5180; Vector Backbone:pLX_TRC931; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30971695
Proper citation: RRID:Addgene_113591 Copy
Genetic Insert: Hybrid Promoter : Plas_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Proper citation: RRID:Addgene_113595 Copy
Genetic Insert: Hybrid Promoter : Plux_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113594 Copy
Genetic Insert: Hybrid Promoter : Prhl_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113593 Copy
Species: E. coli
Genetic Insert: Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pCas; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113633 Copy
Species: Homo sapiens
Genetic Insert: p97
Vector Backbone Description: Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113510 Copy
Genetic Insert: mKaxxte2.5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30804405
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
Proper citation: RRID:Addgene_113630 Copy
Species: Homo sapiens
Genetic Insert: I3
Vector Backbone Description: Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113515 Copy
Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pDAI-SceI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25172289
Comments: Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function
Proper citation: RRID:Addgene_113635 Copy
Vector Backbone Description: Backbone Size:6775; Vector Backbone:pMLBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Trimethoprim
Defining Citation: PMID:15925406
Proper citation: RRID:Addgene_113634 Copy
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113518 Copy
Species: Mus musculus
Genetic Insert: I-Ab beta Derp1-117
Vector Backbone Description: Backbone Size:3818; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding alpha chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes. The antigenic peptide sequence (residues 117-127 of Der p 1 allergen) is encoded as a fusion to the N-terminus of I-Ab beta, connected via a Gly-Ser linker. This fusion is encoded just downstream of the signal peptide, which should be cleaved during post-translational processing. Alternative antigenic peptides can be cloned in place of Der p 1 117-127 by exploiting the XmaI and SpeI restriction sites flanking this sequence.
Proper citation: RRID:Addgene_113639 Copy
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113517 Copy
Species: Mus musculus
Genetic Insert: I-Ab alpha BirA
Vector Backbone Description: Backbone Size:3808; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding beta chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes.
Proper citation: RRID:Addgene_113638 Copy
Genetic Insert: TBG promoter, Top tumor suppressor gRNAs
Vector Backbone Description: Vector Backbone:TBG-Vector (Addgene #109396); Vector Types:Mammalian Expression, Lentiviral, AAV, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29503867
Proper citation: RRID:Addgene_113585 Copy
Species: Synthetic
Genetic Insert: EJ7-GFP variant of EGFP
Vector Backbone Description: Vector Backbone:pCMV6-AC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29950655
Proper citation: RRID:Addgene_113617 Copy
Species: Synthetic
Genetic Insert: NIR-GECO1
Vector Backbone Description: Backbone Size:6160; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30664778
Proper citation: RRID:Addgene_113680 Copy
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