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Species: Aedes aegypti
Genetic Insert: AAEL017335 CAPA R
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115409 Copy
Species: A. aeolicus
Genetic Insert: tRNA methyltransferase
Vector Backbone Description: Backbone Size:6520; Vector Backbone:pHM6g; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14517984
Comments: Please note that the plasmid pHM6g.AQ_1489 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11539 Copy
Species: M. jannaschii
Genetic Insert: phosphodiesterase
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pLIC.B2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15128743
Comments: Please note that the plasmid pLIC.B2.MJ0936 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1154 Copy
Species: bacteriophage P1
Genetic Insert: Phosphoglycerate kinase 1 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2906; Vector Backbone:XhopKS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Full Gene Name: PGK-Cre-bpA: Phosphoglycerate kinase I promoter (PGK), the bacteriophage P1 recombinase Cre with a SV40 large T antigen nuclear localization signal (NLS-Cre), bovine growth hormone polyadenylation site (bpA). Use to express the Cre recombinase in mammalian cells. The vector backbone is a variant of pBluescriptKS- (Stratagene), in which the Xbal site was converted to an Xhol site (provided by Paul Hasty) (Soriano P., Cell 1997). The plasmid was generated by Kurt Fellenberg. He cloned a NLS-Cre sequence, as published by Hua Gu (Gu H, Zou YR, Rajewsky K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164) and cloned it into the pPGK-neo-bpA (Soriano P, Montgomery C, Geske R, Bradley A. 1991 Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell. Feb 22;64(4):693-702.), replacing the neomycin resistance gene (neo) with the NLS-Cre.
NOTE: There are some discrepancies between the Addgene quality control sequence and the sequence provided by the depositing lab, but these differences should not affect plasmid function.
Proper citation: RRID:Addgene_11543 Copy
Species: Homo sapiens
Genetic Insert: GNB2 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Comments: Note: Plasmid contains a GCC deletion and a T to C change in the GNB2 5'UTR. These changes are not known to affect plasmid function.
Proper citation: RRID:Addgene_115355 Copy
Species: Homo sapiens
Genetic Insert: TAK1 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Comments: Note: Plasmid contains an A to G nucleotide change within the TAK1 5'UTR. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_115356 Copy
Species: Homo sapiens
Genetic Insert: shMBNL3-0455
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115463 Copy
Species: Homo sapiens
Genetic Insert: shMBNL3-9465
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115464 Copy
Species: Homo sapiens
Genetic Insert: shMBNL2-3712
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115461 Copy
Species: Homo sapiens
Genetic Insert: shMBNL2-1082
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115462 Copy
Species: Synthetic
Genetic Insert: Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
Vector Backbone Description: Vector Backbone:pOGG026; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30692983
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_115505 Copy
Species: Synthetic
Genetic Insert: Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
Vector Backbone Description: Vector Backbone:pOGG026; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30692983
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_115506 Copy
Species: Synthetic
Genetic Insert: celB pOGG050, T-pharma pOGG003 assembled in pOGG026
Vector Backbone Description: Vector Backbone:pOGG026; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30692983
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_115507 Copy
Species: Homo sapiens
Genetic Insert: shSNAI1
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115467 Copy
Species: Rattus norvegicus
Genetic Insert: Farp1
Vector Backbone Description: Vector Backbone:pCAG-BGH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23209303
Proper citation: RRID:Addgene_115501 Copy
Genetic Insert: myristoylated GFP
Vector Backbone Description: Backbone Marker:Jun-ichi Miyazaki; Vector Backbone:pCAG-GS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20368431
Proper citation: RRID:Addgene_115502 Copy
Species: Synthetic
Genetic Insert: pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30692983
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_115503 Copy
Species: Synthetic
Genetic Insert: sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
Vector Backbone Description: Vector Backbone:pOGG026; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30692983
Comments: Reporter Plasmid constructed with constitutive promoter pNeo (pOGG001) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026 . Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_115509 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SPT2
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30804216
Comments: Please visit https://www.biorxiv.org/content/10.1101/345637v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_115573 Copy
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