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Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Proper citation: RRID:Addgene_113070 Copy
Species: Homo sapiens
Genetic Insert: prostatic acid phosphatase isoform 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233530
Comments: The plasmid contains a V360A compared to the reference NP_001090.2 that is not of concern for function.
Proper citation: RRID:Addgene_113052 Copy
Species: Homo sapiens
Genetic Insert: human Kv2.1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113056 Copy
Genetic Insert: 2 bulged bind sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12600936
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
AAATGAACAA TAA T TCTAGA GCGGCCGCT.
We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.
This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.
All UTR inserts were then constructed in the following format:
TCTAGA CTCGAGCCGG[binding site]ATCGC GGGCCC. A single perfect site
sequence is AAGTTTTCACTCCAGCTAACACCGG.
CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
Proper citation: RRID:Addgene_11306 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5000; Vector Backbone:pZDrRosa26; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Comments: Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_113163 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5500; Vector Backbone:pZDrRosa26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Comments: Note: Addgene NGS unable to fully resolve the CAG promoter sequence. Please refer to the depositor's provided sequence for that section of the plasmid.
Proper citation: RRID:Addgene_113162 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA for rat Rosa26
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:9271; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Comments: Note: Addgene NGS unable to fully resolve the CAG promoter sequence. Please refer to the depositor's provided sequence for that section of the plasmid.
Proper citation: RRID:Addgene_113161 Copy
Vector Backbone Description: Backbone Marker:IDT; Backbone Size:1900; Vector Backbone:pIDTKan; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113160 Copy
Species: Homo sapiens
Genetic Insert: Omomyc
Vector Backbone Description: Backbone Marker:David Turner; Vector Backbone:pCS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9824157
Proper citation: RRID:Addgene_113164 Copy
Vector Backbone Description: Backbone Size:5189; Vector Backbone:pDM304; Vector Types:Dictyostelium discoideum integrating expression vector, C-terminal mScarlet tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Proper citation: RRID:Addgene_113208 Copy
Vector Backbone Description: Backbone Size:9641; Vector Backbone:pDM1038; Vector Types:Dictyostelium discoideum inducible expression vector, N-terminal mTurquoise2 tagging; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113205 Copy
Species: Homo sapiens
Genetic Insert: Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen)
Vector Backbone Description: Backbone Marker:David Turner; Vector Backbone:pCS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113170 Copy
Species: Rattus norvegicus
Genetic Insert: Two gRNAs for rat Rosa26
Vector Backbone Description: Backbone Marker:NIDA OTTC; Backbone Size:6000; Vector Backbone:pOTTC407; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113156 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA for rat TH
Vector Backbone Description: Backbone Marker:NIDA OTTC; Backbone Size:6000; Vector Backbone:pOTTC407; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113159 Copy
Species: S. pyogenes
Genetic Insert: E. coli CRISPR array
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Integration substrate; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27595346
Proper citation: RRID:Addgene_113252 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113240 Copy
Species: E. coli
Genetic Insert: IHFa F13A
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113244 Copy
Species: S. pyogenes
Genetic Insert: S. pyogenes Cas1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pET16bMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27595346
Proper citation: RRID:Addgene_113248 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11317 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11318 Copy
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