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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pHIV-Luciferase
 
Resource Report
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10+ mentions
RRID:Addgene_21375 Ampicillin The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published. NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function. Backbone Size:8713; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:10:05 31
pHIV-EGFP
 
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50+ mentions
RRID:Addgene_21373 Ampicillin PMID:18371425 HIV-EGFP is a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with EGFP. This construct was described in the publication: Cell Stem Cell. 2008 Jan 10. 2(1):90-102. Backbone Size:6900; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:10:16 62
pCM1.26
 
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RRID:Addgene_21377 L3787 promoter::GFP in pDONRP4P1R Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:05 0
M-PKD2 (OF2-3)
 
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1+ mentions
RRID:Addgene_21370 autosomal dominant polycystic kidney disease type II Homo sapiens Ampicillin PMID:11140688 *Note that this construct has a rare SNP (rs1131408) at position 4:88967820 that results in a missense change (G449V). The polymorphism is not known to affect function. Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin wild-type* 2026-08-01 01:10:16 4
pGL3-VEGFR2-225
 
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1+ mentions
RRID:Addgene_21306 VEGFR2 promoter Homo sapiens Ampicillin PMID:19242469 -225 to +268 region of the promoter. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:10:04 1
pGL3-VEGFR2-570
 
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RRID:Addgene_21309 VEGFR2 promoter Homo sapiens Ampicillin PMID:19242469 -570 to +268 region of the promoter. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:10:16 0
pCM1.128
 
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RRID:Addgene_21385 spe-11 promoter (no ATG) in pDONRP4P1R Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:17 0
pCM1.127
 
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1+ mentions
RRID:Addgene_21384 pie-1 promoter (short, no ATG) in pDONRP4P1R Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:05 5
pAc5.1C-FLuc-Stop-5BoxB
 
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1+ mentions
RRID:Addgene_21301 5BoxB Ampicillin PMID:16177138 Backbone Marker:Elisa Izaurralde; Backbone Size:7184; Vector Backbone:pAc5.1C-FLuc; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin 2026-08-01 01:10:04 4
pCM3.23
 
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RRID:Addgene_21389 gld-1 ORF+3' in pDONR201 Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:05 0
CFP-C1-PLCdelta-PH
 
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1+ mentions
RRID:Addgene_21262 PLCdelta Homo sapiens Kanamycin PMID:11134066 Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin PH domain of PLCdelta (human). Also known as PLCdelta-PH-CFP. Lab plasmid WC0026. 2026-08-01 01:10:04 4
pCM1.122
 
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RRID:Addgene_21383 msp-56 promoter in pDONRP4P1R Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:05 0
Krox20-MSE8 pGL3-TATA
 
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1+ mentions
RRID:Addgene_21261 Krox20-Myelin Specific Enhancer Mus musculus Ampicillin PMID:19179536 Plasmid 21261: Krox20-MSE8 pGL3-TATA sequence match with genomic sequences on Chromosome 10 upstream of Egr2. It's an enhancer of Egr2 localized ~35 kb downstream of the defined genomic sequence of Egr2. If comparing the genomic location of plasmid 21261 (Chromosome 10: 9367948-9368511) and plasmid 21260 (Chromosome 10: 9331837-9332418), one can see that it's approximately 35 kb apart. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:10:15 2
pCM1.108
 
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RRID:Addgene_21382 him-3 prom in PDONRP4P1R Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:17 0
Krox20 promoter pGL3-TATA
 
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1+ mentions
RRID:Addgene_21260 Krox20 promoter Mus musculus Ampicillin PMID:19179536 There is a T to C mismatch with Krox20. The single mis-match of the nucleotide is likely to reflect the polymorphism of different mouse strain used in the cloning. Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:10:04 2
pCM1.95
 
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RRID:Addgene_21381 lip-1 M1M2 3'utr in pDONRP2RP3 Caenorhabditis elegans Kanamycin PMID:18818082 Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:10:05 0
pLenti CMV V5-LUC Blast (w567-1)
 
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10+ mentions
RRID:Addgene_21474 V5-LUC Firefly Ampicillin PMID:19657394 Backbone Size:7687; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:10:05 29
pLenti X2 Puro/pTER shLUC (w589-1)
 
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1+ mentions
RRID:Addgene_21472 Firefly luciferase shRNA Ampicillin PMID:19657394 Backbone Size:7757; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-01 01:10:05 1
pLenti PGK V5-LUC Neo (w623-2)
 
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10+ mentions
RRID:Addgene_21471 V5-LUC Firefly Ampicillin PMID:19657394 Backbone Size:7334; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin 2026-08-01 01:10:17 37
pBS Notch4HA
 
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RRID:Addgene_21470 Notch4 Mus musculus Ampicillin PMID:9576833 Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSKS; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-01 01:10:05 0

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