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Vector Backbone Description: Backbone Size:8713; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published.
NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.
Proper citation: RRID:Addgene_21375 Copy
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371425
Comments: HIV-EGFP is a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with EGFP. This construct was described in the publication: Cell Stem Cell. 2008 Jan 10. 2(1):90-102.
Proper citation: RRID:Addgene_21373 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3787 promoter::GFP in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21377 Copy
Species: Homo sapiens
Genetic Insert: autosomal dominant polycystic kidney disease type II
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11140688
Comments: *Note that this construct has a rare SNP (rs1131408) at position 4:88967820 that results in a missense change (G449V). The polymorphism is not known to affect function.
Proper citation: RRID:Addgene_21370 Copy
Species: Homo sapiens
Genetic Insert: VEGFR2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19242469
Comments: -225 to +268 region of the promoter.
Proper citation: RRID:Addgene_21306 Copy
Species: Homo sapiens
Genetic Insert: VEGFR2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19242469
Comments: -570 to +268 region of the promoter.
Proper citation: RRID:Addgene_21309 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 promoter (no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21385 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 promoter (short, no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21384 Copy
Genetic Insert: 5BoxB
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:7184; Vector Backbone:pAc5.1C-FLuc; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16177138
Proper citation: RRID:Addgene_21301 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21389 Copy
Species: Homo sapiens
Genetic Insert: PLCdelta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11134066
Proper citation: RRID:Addgene_21262 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-56 promoter in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21383 Copy
Species: Mus musculus
Genetic Insert: Krox20-Myelin Specific Enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Comments: Plasmid 21261: Krox20-MSE8 pGL3-TATA sequence match with genomic sequences on Chromosome 10 upstream of Egr2. It's an enhancer of Egr2 localized ~35 kb downstream of the defined genomic sequence of Egr2. If comparing the genomic location of plasmid 21261 (Chromosome 10: 9367948-9368511) and plasmid 21260 (Chromosome 10: 9331837-9332418), one can see that it's approximately 35 kb apart.
Proper citation: RRID:Addgene_21261 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-3 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21382 Copy
Species: Mus musculus
Genetic Insert: Krox20 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Comments: There is a T to C mismatch with Krox20. The single mis-match of the nucleotide is likely to reflect the polymorphism of different mouse strain used in the cloning.
Proper citation: RRID:Addgene_21260 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21381 Copy
Species: Firefly
Genetic Insert: V5-LUC
Vector Backbone Description: Backbone Size:7687; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_21474 Copy
Genetic Insert: Firefly luciferase shRNA
Vector Backbone Description: Backbone Size:7757; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_21472 Copy
Species: Firefly
Genetic Insert: V5-LUC
Vector Backbone Description: Backbone Size:7334; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_21471 Copy
Species: Mus musculus
Genetic Insert: Notch4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSKS; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9576833
Proper citation: RRID:Addgene_21470 Copy
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