Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Cloning vector
Genetic Insert: ECFPpA-ACN
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2394; Vector Backbone:pSP72; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18546598
Proper citation: RRID:Addgene_20301 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: GAL1,10;
X: UbiY;
Linker: none
Proper citation: RRID:Addgene_20389 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: GAL1,10;
X: UbiM;
Linker: none
Proper citation: RRID:Addgene_20388 Copy
Genetic Insert: mir21(P) targets
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12554881
Proper citation: RRID:Addgene_20382 Copy
Species: E. coli
Genetic Insert: Pmyc1tetO-lacZ
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20315 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
Genetic Insert: YFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: YFP-reporter;
UAS: ADH1;
X: M;
Linker:none
Proper citation: RRID:Addgene_20397 Copy
Species: Mus musculus
Genetic Insert: Sirt6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15795229
Proper citation: RRID:Addgene_20275 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: FUS1;
X:UbiM;
Linker:delta k
Proper citation: RRID:Addgene_20399 Copy
Species: Mus musculus
Genetic Insert: Sirt6
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15795229
Proper citation: RRID:Addgene_20277 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: GAL1,10;
X: UbiE;
Linker: ek
Proper citation: RRID:Addgene_20393 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: GAL1,10;
X:UbiE;
Linker:delta k
Proper citation: RRID:Addgene_20402 Copy
Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Ezaz-Nikpay et al, 1994 Protein Science; Backbone Size:3531; Vector Backbone:pKEN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8707053
Comments: An XbaI site was introduced at the beginning of GFP, see attached map for more details.
The protein is expressed from IPTG inducible promoter in pKEN vector. GFP is cloned as XbaI-PstI fragment into the polylinker; there is also NdeI site at the AUG that can be used.
Mut 2 is probably best for expression in bacteria.
Overexpression of GFP renders the cells osmosensitive. In the absence of IPTG, DH12 carrying the enclosed plasmids will lyse in LB. We recommend growing this plasmid in low salt LB (<5g/L NaCl); alternatively use E.coli strain that grossly overproduces lacI and totally shuts expression down.
Proper citation: RRID:Addgene_20409 Copy
Species: Rattus norvegicus
Genetic Insert: AnxA6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18850003
Comments: AnxA6 was cut out of pGEMT with HindIII and XbaI and ligated into pcDNA3.1 + with HindIII and XbaI
Proper citation: RRID:Addgene_20407 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS8
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20247 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO-conjugating enzyme Ubc9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_20368 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS7
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20246 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS5
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20244 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin B +1 transcript
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18796559
Proper citation: RRID:Addgene_20254 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19139065
Comments: TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.
NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.
Proper citation: RRID:Addgene_20375 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
