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Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18397888
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
Proper citation: RRID:Addgene_25071 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18397888
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
The domain structure and amino acid limits is approximate. According to depositor's domain organization, this construct contains the correct residues.
Proper citation: RRID:Addgene_25070 Copy
Species: Plasmodium falciparum
Genetic Insert: PF14_0309:C34112
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 2PBF. http://www.thesgc.org/structures/2PBF/ .
Proper citation: RRID:Addgene_25106 Copy
Species: Cryptosporidium parvum
Genetic Insert: cgd2_4120:C33348
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 2POY. http://www.thesgc.org/structures/2POY/ .
Proper citation: RRID:Addgene_25109 Copy
Species: Homo sapiens
Genetic Insert: pro-SUMO3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17591783
Proper citation: RRID:Addgene_25103 Copy
Species: Homo sapiens
Genetic Insert: pro-SUMO2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17591783
Proper citation: RRID:Addgene_25102 Copy
Species: Cryptosporidium parvum
Genetic Insert: Cp-PF08_0120:C31326
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 2OWA. http://www.thesgc.org/structures/2OWA/ .
Proper citation: RRID:Addgene_25105 Copy
Species: Homo sapiens
Genetic Insert: RanGAP1 deleted N-term domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17591783
Proper citation: RRID:Addgene_25104 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18397888
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
Proper citation: RRID:Addgene_25065 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18397888
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
The domain structure and amino acid limits is approximate. According to depositor's domain organization, this construct contains the correct residues.
Proper citation: RRID:Addgene_25068 Copy
Species: Mus musculus
Genetic Insert: ytotoxic T-murine Lymphocyte Antigen 4
Vector Backbone Description: Backbone Size:7337; Vector Backbone:pAL119; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12515725
Proper citation: RRID:Addgene_25100 Copy
Species: Homo sapiens
Genetic Insert: BimL (S58D,DE)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12591950
Proper citation: RRID:Addgene_25061 Copy
Species: Homo sapiens
Genetic Insert: BimL (AAA)
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12591950
Proper citation: RRID:Addgene_25063 Copy
Species: Homo sapiens
Genetic Insert: CBX2_04
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid # 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: For more information, please see:
http://www.thesgc.org/structures/3H91/
http://www.thesgc.org/structures/5EPK/
Proper citation: RRID:Addgene_25158 Copy
Species: Homo sapiens
Genetic Insert: dpp03.001.724
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:0; Vector Backbone:pFHMSP LIC-N; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3FVY. http://www.thesgc.org/structures/3FVY/
Proper citation: RRID:Addgene_25154 Copy
Species: Homo sapiens
Genetic Insert: KRASb:M1-K169
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3GFT. http://www.thesgc.org/structures/3GFT/
Proper citation: RRID:Addgene_25153 Copy
Species: Homo sapiens
Genetic Insert: MBD4_12
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3IHO. http://www.thesgc.org/structures/3IHO/
Proper citation: RRID:Addgene_25155 Copy
Species: Homo sapiens
Genetic Insert: nedd4l.0574.0947
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2ONI. http://www.thesgc.org/structures/2ONI/
Proper citation: RRID:Addgene_25169 Copy
Species: Homo sapiens
Genetic Insert: ubh12.126.285
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3DB3. http://www.thesgc.org/structures/3DB3/
Proper citation: RRID:Addgene_25164 Copy
Species: Homo sapiens
Genetic Insert: CYP51A1_cw_09
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:6980; Vector Backbone:pCW-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB ID: 3LD6 (supersedes 3I3K) https://www.rcsb.org/structure/3LD6
SGC link: https://www.thesgc.org/structures/3I3K/
Proper citation: RRID:Addgene_25161 Copy
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