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Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA-4C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19553310
Proper citation: RRID:Addgene_24744 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24864 Copy
Species: HPV31
Genetic Insert: HPV31 E2 (HA)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4141; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20181716
Proper citation: RRID:Addgene_24740 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24860 Copy
Species: HPV31
Genetic Insert: HPV31 KWK E8^E2C (HA)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4141; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20181716
Proper citation: RRID:Addgene_24742 Copy
Species: HPV31
Genetic Insert: HPV31 E8^E2C (HA)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4141; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20181716
Proper citation: RRID:Addgene_24741 Copy
Species: Homo sapiens
Genetic Insert: Sirt3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5075; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18794531
Comments: Catalytically inactive mutant
Proper citation: RRID:Addgene_24917 Copy
Species: N/A
Genetic Insert: CAT-ccdB cassette
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 24754; Backbone Size:1851; Vector Backbone:pAsylum; Vector Types:Gateway-adapted entry vector; Bacterial Resistance:Chloramphenicol and Bleocin (Zeocin)
Defining Citation: PMID:20542254
Proper citation: RRID:Addgene_24755 Copy
Species: Homo sapiens
Genetic Insert: Rabin8
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24910 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24870 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24738 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24859 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24735 Copy
Species: Homo sapiens
Genetic Insert: BRaf-ER
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS no LacZ; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11896581
Proper citation: RRID:Addgene_24949 Copy
Species: Mus musculus
Genetic Insert: IL-10 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10657644
Proper citation: RRID:Addgene_24948 Copy
Species: Mus musculus
Genetic Insert: IL-10 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10657644
Comments: There are 3 point mutations in the promoter, but they should not affect activity.
Proper citation: RRID:Addgene_24942 Copy
Species: Mus musculus
Genetic Insert: IL-10 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10657644
Proper citation: RRID:Addgene_24944 Copy
Species: Mus musculus
Genetic Insert: Ezh2
Vector Backbone Description: Backbone Size:7000; Vector Backbone:MSCV hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20368440
Proper citation: RRID:Addgene_24927 Copy
Species: Homo sapiens
Genetic Insert: GADD45
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10973963
Comments: Plasmid size: 5867 bp
The HA sequence is between XbaI and ApaI restriction enzyme sites.
Proper citation: RRID:Addgene_24929 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7767; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16750377
Proper citation: RRID:Addgene_25050 Copy
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