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Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24803 Copy
Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24802 Copy
Species: Homo sapiens
Genetic Insert: LC3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18296641
Proper citation: RRID:Addgene_24920 Copy
Genetic Insert: NpuDnaE-IntC
Vector Backbone Description: Backbone Size:3793; Vector Backbone:pRSF-1b (Invitrogen); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20203672
Proper citation: RRID:Addgene_24765 Copy
Species: Homo sapiens
Genetic Insert: TGFbeta receptor II
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: HA tag: PYDVPDYA
Proper citation: RRID:Addgene_24801 Copy
Species: Homo sapiens
Genetic Insert: Atg5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296641
Proper citation: RRID:Addgene_24922 Copy
Species: Homo sapiens
Genetic Insert: Atg7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296641
Proper citation: RRID:Addgene_24921 Copy
Species: Homo sapiens
Genetic Insert: F-CDK8L
Vector Backbone Description: Backbone Marker:Addgene plasmid 15439; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15680972
Proper citation: RRID:Addgene_24762 Copy
Species: Homo sapiens
Genetic Insert: Pirh2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX-2TKm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043414
Comments: E. coli strain for expression: BL21DE3
Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers.
Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours.
For pGEX-2TKm vector, the kinase site RRASV is removed and NdeI site is inserted at 5' of BamHI.
Proper citation: RRID:Addgene_24882 Copy
Species: Mus musculus
Genetic Insert: Zinc Finger Protein 423
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCV puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20200519
Proper citation: RRID:Addgene_24764 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18568018
Proper citation: RRID:Addgene_24814 Copy
Species: Homo sapiens
Genetic Insert: hRab8A
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pDEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24898 Copy
Species: Bacterial
Genetic Insert: TetR
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15933004
Comments: There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector.
Infect Immun. 2001 Dec;69(12):7851-7.
Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation.
Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL.
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Abstract
An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB.
PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl
Proper citation: RRID:Addgene_24779 Copy
Species: Homo sapiens
Genetic Insert: hRab8A[T22N]
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pDEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24899 Copy
Species: Homo sapiens
Genetic Insert: p35
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11813240
Proper citation: RRID:Addgene_24895 Copy
Species: Danio rerio
Genetic Insert: Rab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24908 Copy
Species: Danio rerio
Genetic Insert: fishRab8B
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Comments: This construct was generated by gene synthesis and is based on a reference sequence from 2007. Amino acids NE 178-179 are missing compared to current NCBI sequences.
Proper citation: RRID:Addgene_24907 Copy
Species: Homo sapiens
Genetic Insert: shRNA-GFP
Vector Backbone Description: Backbone Marker:Halo-Bio RNAi Therapeutics; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16611886
Comments: shRNA oligo sequence is - 5′-GCAAGCTGACCCTGAAGTTC
Proper citation: RRID:Addgene_24747 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24868 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24749 Copy
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