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Species: Xenopus laevis
Genetic Insert: cerberus
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10067895
Comments: For injection cut with NotI.
Transcribe with SP6.
The construct fuses aa 1-25 (KHSEG25) to aa 117-270 (117RRKA)
Proper citation: RRID:Addgene_24681 Copy
Species: Xenopus laevis
Genetic Insert: twisted gastrulation
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10866189
Comments: Best for in situs
For sense injection:
Cut with BamHI
Transcribe with SP6
Proper citation: RRID:Addgene_24680 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Size:4873; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
Four novel mutations in the OFD1 (Cxorf5) gene in Finnish patients with oral-facial-digital syndrome 1.
Rakkolainen A, Ala-Mello S, Kristo P, Orpana A, Järvelä I.
J Med Genet. 2002 Apr;39(4):292-6. No abstract available. PMID: 11950863
Proper citation: RRID:Addgene_24575 Copy
Species: Xenopus laevis
Genetic Insert: secreted frizzled-related sequence protein 2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10960783
Comments: For sense linearize with NotI, transcribe with SP6.
Proper citation: RRID:Addgene_24696 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Size:4873; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.
Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.
J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504
Proper citation: RRID:Addgene_24574 Copy
Species: Xenopus laevis
Genetic Insert: Crescent
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10960783
Comments: For sense linearize with NotI, transcribe with SP6.
Proper citation: RRID:Addgene_24697 Copy
Species: C. glutamicum
Genetic Insert: TetRO promoter
Vector Backbone Description: Backbone Size:6078; Vector Backbone:pSHKLx; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15687380
Proper citation: RRID:Addgene_24730 Copy
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pBR322; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6835398
Comments: This plasmid is a derivative of pBR322. This plasmid contains a deletion from Hae II site 778 to Hae II site 2352. A polylinker has been added incorporating the Hind III and Cla I sites of pBR322. 4 bp of the EcoRI have been deleted.
Proper citation: RRID:Addgene_24571 Copy
Species: Homo sapiens
Genetic Insert: WNK2`
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1/V5–His–TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17578925
Proper citation: RRID:Addgene_24570 Copy
Species: Xenopus laevis
Genetic Insert: Frzb-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10960783
Comments: For antisense linearize with HindIII, transcribe with T7
For sense linearize with NotI, transcribe with SP6
Proper citation: RRID:Addgene_24694 Copy
Species: Xenopus laevis
Genetic Insert: DAN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16172975
Comments: For antisense linearize with EcoRI, transcribe with T7
For sense linearize with NotI, transcribe with SP6
Proper citation: RRID:Addgene_24693 Copy
Species: Homo sapiens
Genetic Insert: human XIST
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2686; Vector Backbone:puc18; Vector Types:plasmid cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8636206
Proper citation: RRID:Addgene_24690 Copy
Species: Bacterial
Genetic Insert: PAg85a-TetR
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pMV206; Vector Types:Mycobacterial shuttle vector; Bacterial Resistance:Kanamycin
Comments: There are a few mismatches between depositor's sequence and the quality control sequence Addgene has obtained. The region of mismatch and gaps is derived from the parental vector and not the important part of the vector.
Infect Immun. 2001 Dec;69(12):7851-7.
Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation.
Bateman BT, Donegan NP, Jarry TM, Palma M, Cheung AL.
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Abstract
An inducible promoter system provides a powerful tool for studying the genetic basis for virulence. A variety of inducible systems have been used in other organisms, including pXyl-xylR-inducible promoter, the pSpac-lacI system, and the arabinose-inducible P(BAD) promoter, but each of these systems has limitations in its application to Staphylococcus aureus. In this study, we demonstrated the efficacy of a tetracycline-inducible promoter system in inducing gene expression in S. aureus in vitro and inside epithelial cells as well as in an animal model of infection. Using the xyl/tetO promoter::gfp(uvr) fusion carried on a shuttle plasmid, we demonstrated that dose-dependent tetracycline induction, as measured by bacterial fluorescence, occurred in each of the above environments while basal activation under noninduced conditions remained low. To ascertain how the system can be used to elucidate the genetic basis of a pathogenic phenotype, we cloned the sigB gene downstream of the inducible promoter. Induction of SigB expression led to dose-dependent attachment of the tested strain to polystyrene microtiter wells. Additionally, bacterial microcolony formation, an event preceding mature biofilm formation, also increased with tetracycline induction of SigB.
PMID: 11705967 [PubMed - indexed for MEDLINE]PMCID: PMC98881Free PMC Articl
Proper citation: RRID:Addgene_24784 Copy
Species: synthetic
Genetic Insert: Prb
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24785 Copy
Genetic Insert: Ptet-Gfp-ASV
Vector Backbone Description: Backbone Size:0; Vector Backbone:pFLAME4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15933004
Comments: 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed]
2.
2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]
Proper citation: RRID:Addgene_24782 Copy
Genetic Insert: Ptet-Gfp-LAA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pFLAME3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15933004
Comments: 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed]
2.
2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]
Proper citation: RRID:Addgene_24781 Copy
Species: synthetic
Genetic Insert: PdarC1
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pCTCON2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24835 Copy
Species: Pyrococcus horikoshii
Genetic Insert: PH1109
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458342
Proper citation: RRID:Addgene_24830 Copy
Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24806 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18568018
Proper citation: RRID:Addgene_24809 Copy
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