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Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.
Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.
J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504
Proper citation: RRID:Addgene_24580 Copy
Species: Homo sapiens
Genetic Insert: TEAD-4
Vector Backbone Description: Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20123905
Comments: This plasmid is identical to plasmid #33039: pRK5-Myc-TEAD4
Proper citation: RRID:Addgene_24638 Copy
Species: Homo sapiens
Genetic Insert: Yap2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24637 Copy
Species: Peptides
Genetic Insert: CB1-CKKp
Vector Backbone Description: Backbone Marker:PMID: 12864922; Backbone Size:0; Vector Backbone:ventx vector; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19472343
Comments: CB1 and the CKKp are the peptides PPPALPPKKR and VKLIPSWTTVILVKSMLRKRSFGNPF, respectively. All the components (Venus, CB1 and CKKp) are linked by an amino acid linker 'AS'.
Proper citation: RRID:Addgene_24597 Copy
Species: E. coli
Genetic Insert: fadD
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20111002
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24630 Copy
Species: Peptides
Genetic Insert: IE-LM
Vector Backbone Description: Backbone Marker:PMID: 12864922; Backbone Size:0; Vector Backbone:pCfvtx; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20167369
Comments: IE peptide: RAAITVQRYVRGYDIKEKLCYVALD
LM peptide:
RIRKKLRKNFIAVSAANRFKKI
Proper citation: RRID:Addgene_24596 Copy
Species: E. coli
Genetic Insert: fadD (M3351), atfA
Vector Backbone Description: Backbone Size:0; Vector Backbone:ColE1?; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20111002
Comments:
single operon, fadD (e.coli) and atfA wax ester synthase
EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24632 Copy
Species: Mus musculus
Genetic Insert: Par6c
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17060907
Proper citation: RRID:Addgene_24598 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FAA2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20111002
Proper citation: RRID:Addgene_24631 Copy
Species: codon optimized cDNA
Genetic Insert: Cre recombinase (codon optimized)
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAAV-pgk-MCS; Vector Types:AAV, Cre/Lox, Adeno-associated; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24593 Copy
Species: Rattus norvegicus
Genetic Insert: PKCZeta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657501
Proper citation: RRID:Addgene_24608 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24605 Copy
Species: polyomavirus
Genetic Insert: VP1 from Human Polyomavirus 7
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 22517; Backbone Size:5388; Vector Backbone:pGwf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:20542254
Proper citation: RRID:Addgene_24726 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24604 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24607 Copy
Species: polyomavirus
Genetic Insert: Full genome of Human polyomavirus 7
Vector Backbone Description: Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:20542254
Comments: genome can be liberated by digestion with Mfe I.
Proper citation: RRID:Addgene_24728 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24606 Copy
Species: Homo sapiens
Genetic Insert: Scribble
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLNCX2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19041750
Proper citation: RRID:Addgene_24600 Copy
Species: Homo sapiens
Genetic Insert: WNK2
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1/V5–His–TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17578925
Proper citation: RRID:Addgene_24569 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16413488
Comments: Best in situ probe.
For anitsense: linearize with NotI
Transcribe with SP6
For mRNA injections use 200 pg to dorsalize.
E248D does not affect function.
Proper citation: RRID:Addgene_24684 Copy
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