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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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PKC eta dNPS Resource Report Resource Website |
RRID:Addgene_21245 | PKC eta | Mus musculus | Ampicillin | PMID:12794082 | Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440. | Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | delta 1-165 | 2026-08-01 01:10:15 | 0 |
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PKC epsilon WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_21240 | PKC epsilon | Mus musculus | Ampicillin | PMID:12794082 | Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:04 | 2 | ||
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GFP-C1(2)delta Resource Report Resource Website 1+ mentions |
RRID:Addgene_21216 | GFP-N2-PKCdelta-C1 | Rattus norvegicus | Kanamycin | PMID:11509231 | Backbone Marker:Clontech; Backbone Size:4596; Vector Backbone:N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | C1(2) domains of PKC delta (rat) (Lab plasmid WC0001) | 2026-08-01 01:10:03 | 6 | |
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KW1 Resource Report Resource Website |
RRID:Addgene_21211 | Mek2 | Homo sapiens | Kanamycin | PMID:15342384 | Constitutive active protein has the following aa sequence deleted - EQQKKRLE, residues 48 to 55. | Backbone Marker:Invitrogen; Backbone Size:2200; Vector Backbone:pENTR1A; Vector Types:Subclone vector; Bacterial Resistance:Kanamycin | KW71, constitutive active | 2026-08-01 01:10:15 | 0 |
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PGK-H2BeGFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_21210 | Phosphoglycerate kinase promoter | Homo sapiens | Ampicillin | PMID:19352491 | There are several mismatches between depositor's and Addgene sequence. These mutations do not affect eGFP expression. | Backbone Size:7226; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:03 | 13 | |
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pCS2-mqgDUX4 Resource Report Resource Website |
RRID:Addgene_21176 | DUX4 | Homo sapiens | Ampicillin | PMID:19359275 | Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:03 | 0 | ||
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GFP-C2gamma Resource Report Resource Website 1+ mentions |
RRID:Addgene_21215 | GFP-pcDNA3-PKCgamma-c2 | Rattus norvegicus | Ampicillin | PMID:11509231 | Backbone Size:5429; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | The C2-domain from PKCgamma (aa170-260) plus flanking sequence. This insert is thus amino acids 158-284 from PKCgamma (rat). (Lab plasmid WC 0005) | 2026-08-01 01:10:03 | 3 | |
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MSCV JMJD3 mutant Resource Report Resource Website 1+ mentions |
RRID:Addgene_21214 | JMJD3 | Homo sapiens | Ampicillin | PMID:18628393 | Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | H1390A | 2026-08-01 01:10:15 | 7 | |
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PM-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_21213 | PM-GFP | Ampicillin | PMID:10598863 | Backbone Size:5419; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | PM targetting sequence is Lyn (MGCIKSKRKD) (Lab plasmid WC 0017) | 2026-08-01 01:10:03 | 9 | ||
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GFP-C1-PLCdelta-PH Resource Report Resource Website 50+ mentions |
RRID:Addgene_21179 | PLCdelta | Homo sapiens | Kanamycin | PMID:9512420 | This construct encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids (Stauffer TP, Ahn S, Meyer T; Curr Biol. 1998 8:343-6). It is best used in combination with a second fluorescent protein that uniformly marks the plasma membrane (the last 20 amino acids "CAAX" region of KRas is often used as a PM marker). Relative changes in cell-wide or local plasma membrane PI(4,5)P2 can be determined by using a mask for the PM region and by dividing the two background subtracted PH domain and CAAX images. At high expression levels, this construct interferes with PI(4,5)P2 signaling and alters cortical actin (Raucher D et al Cell. 2000 100:221-8). | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | PH domain of human PLC delta, amino acids Met1–Ile175. (Lab plasmid WC 0003) | 2026-08-01 01:10:14 | 94 |
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MSCV JMJD3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_21212 | JMJD3 | Homo sapiens | Ampicillin | PMID:18628393 | Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:03 | 9 | ||
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pGL3-Control-E2F-3'UTR-WT Resource Report Resource Website |
RRID:Addgene_21170 | E2F transcription factor 1 | Homo sapiens | Ampicillin | PMID:15944709 | Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:03 | 0 | ||
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pCS2-mkgDUX4mal* Resource Report Resource Website |
RRID:Addgene_21172 | DUX4 | Homo sapiens | Ampicillin | PMID:19359275 | Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | Point mutation at nucleotide 5114 (numbering according to sequence published in corresponding article): tCg to tAg | 2026-08-01 01:10:14 | 0 | |
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pGL3-Control-E2F-3'UTR-Mut Resource Report Resource Website |
RRID:Addgene_21171 | E2F 3'UTR Mutant | Homo sapiens | Ampicillin | PMID:15944709 | Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2 miR binding sites mutated. Changed from GCACTTT to GGAGTGT. | 2026-08-01 01:10:03 | 0 | |
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aMHC-eGFP-Rex-Neo Resource Report Resource Website 1+ mentions |
RRID:Addgene_21229 | alpha myosin heavy chain promoter | Mus musculus | Ampicillin | PMID:19352491 | Backbone Size:8400; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:04 | 9 | ||
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aMHC-mCherry-Rex-Blasticidin Resource Report Resource Website 1+ mentions |
RRID:Addgene_21228 | alpha myosin heavy chain promoter | Mus musculus | Ampicillin | PMID:19352491 | There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct. | Backbone Size:7338; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:10:04 | 5 | |
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pRosa26-DEST Resource Report Resource Website 10+ mentions |
RRID:Addgene_21189 | GateWay conversion cassette | Chloramphenicol and Ampicillin | PMID:19014667 | pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4 for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation. Stbl3 cells are advisable to use after users have done the LR recombination reaction. See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html for more information and protocols for this plasmid. Further advice from the depositing lab: "Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive... - Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay" Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct. | Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-01 01:10:03 | 18 | ||
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GFP-C1-CAMKIIalpha-T286A Resource Report Resource Website 1+ mentions |
RRID:Addgene_21220 | CAMKIIalpha-T286A | Rattus norvegicus | Kanamycin | PMID:10102820 | Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | T286A (Lab plasmid KS 0003). | 2026-08-01 01:10:04 | 1 | |
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GFP-C1-CAMKIIalpha Resource Report Resource Website 10+ mentions |
RRID:Addgene_21226 | CAMKIIalpha | Rattus norvegicus | Kanamycin | PMID:9768845 | Backbone Marker:Clontech; Backbone Size:4725; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Lab plasmid KS 0001. | 2026-08-01 01:10:04 | 10 | |
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pEGFP-C1-CaMKIIbeta(A303R) Resource Report Resource Website |
RRID:Addgene_21224 | CaMKIIbeta(A303R) | Rattus norvegicus | Kanamycin | PMID:10102820 | Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | CaMKIIbeta(A303R) (Lab plasmid CF 0002) | 2026-08-01 01:10:15 | 0 |
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