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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 602-612bp
BsaI site #2 = 2040-2050bp
Proper citation: RRID:Addgene_48869 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 640-650bp
BsaI site #2 = 2078-2088bp
Proper citation: RRID:Addgene_48868 Copy
Species: E. coli
Genetic Insert: ccdB Cassette
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Synthetic Biology, Module 0; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_126062 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141066 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_167299 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUBQ4-2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167690 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUBQ4-2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167693 Copy
Species: Staphylococcus aureus
Genetic Insert: pEC1.2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167692 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167691 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUBQ4-2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167687 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167689 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::Cas9i
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167688 Copy
Species: Staphylococcus aureus
Genetic Insert: PcUB4-2::asCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167681 Copy
Species: Streptococcus pyogenes
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167679 Copy
Species: Streptococcus pyogenes
Genetic Insert: PcUB4-2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: in addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167677 Copy
Species: Synthetic
Genetic Insert: RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
Vector Backbone Description: Backbone Marker:VIB, Ghent Univ, Kamiri et al ; Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Gateway destination vector for plants to express ORFs from entry clone with RecA1 plastid targeting signal under RPS5A promoter.
Proper citation: RRID:Addgene_171723 Copy
Species: Synthetic
Genetic Insert: attR3-CmR-ccdB-attR4
Vector Backbone Description: Backbone Marker:Kan Wang; Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please note that there are minor differences between the Addgene verified sequence and the depositor's reference sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_172181 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pP7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_133747 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen modified by doi:10.1271/bbb.70678; Backbone Size:10835; Vector Backbone:R4pGWB401; Vector Types:Plant Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_128432 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBiFCt-2in1-CC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105114 Copy
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