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Species:
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Hy_pUbi-p63e eGFP SV40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176300 Copy
Species:
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Hy_pMtnA eGFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176302 Copy
Species:
Genetic Insert: RxCas13d
Vector Backbone Description: Vector Backbone:pUb-3xFLAG-MCS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176304 Copy
Species:
Genetic Insert: RxCas13d
Vector Backbone Description: Vector Backbone:pUb-3xFLAG-MCS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176303 Copy
Species: Homo sapiens
Genetic Insert: ZNF608 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176380 Copy
Species: Homo sapiens
Genetic Insert: SOX2 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176382 Copy
Species: Synthetic
Genetic Insert: HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
Vector Backbone Description: Vector Backbone:piggyBac-EF1alpha; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid name in Yang lab: HGpl553
Proper citation: RRID:Addgene_176459 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176452 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-SD1-S477N
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176453 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R-T478K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176456 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R-E484Q
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176455 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-SD1-WA1
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176448 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-K417N-E484K-N501Y
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176440 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-S477N
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176443 Copy
Species:
Genetic Insert: SARS-CoV-2-RBD-L452R-T478K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176446 Copy
Species: Mus musculus
Genetic Insert: hU6-sgCRY1-2
Vector Backbone Description: Backbone Size:5288; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the mutations K14E, N210D, and E249G were found in the mCherry sequence. The fluorophore is still fluorescent, as described in the publication.
Proper citation: RRID:Addgene_176511 Copy
Species: Synthetic
Genetic Insert: HNHx-ABEmax7.10HD
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176477 Copy
Species: Mus musculus
Genetic Insert: dynein regulatory complex subunit 7
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176470 Copy
Species: Other
Genetic Insert: 4x SV40 polyA
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript II SK(-); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176506 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176462 Copy
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