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Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5."
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24501 Copy
Species: Homo sapiens
Genetic Insert: RASSL Ro1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt
Proper citation: RRID:Addgene_24504 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio.
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24500 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684828
Proper citation: RRID:Addgene_24460 Copy
Species: Aequorea victoria and Discosoma sp. reef coral
Genetic Insert: Split RFP-GFP chimera
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20352097
Proper citation: RRID:Addgene_24515 Copy
Species: Aequorea victoria and Discosoma sp. reef coral
Genetic Insert: Split GFP-RFP chimera
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20352097
Proper citation: RRID:Addgene_24514 Copy
Species: Caenorhabditis elegans
Genetic Insert: Unc104
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12351789
Proper citation: RRID:Addgene_24476 Copy
Species: Rattus norvegicus
Genetic Insert: SypHy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3952; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16982422
Comments: The depositing lab notes that the Xba1 and Bcl1 unique restriction digestion sites could be methylated and blocked if the plasmid is grown or maintained in dam+ bacteria.
Also note that in the Addgene-generated map below, the feature listed as "GFP(12-708)" is actually pH sensitive pHluorin.
There is a mutation S446T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.
Proper citation: RRID:Addgene_24478 Copy
Species: Aequorea victoria (Jellyfish)
Genetic Insert: Yn, Yc, ECFP, EYFP
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:7434; Vector Backbone:pVitro2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors.
Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings
Proper citation: RRID:Addgene_24510 Copy
Species: reporter gene Venus
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10474; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24568 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24443 Copy
Species: reporter gene eGFP
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10444; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24566 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24560 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Comments: Note: this plasmid is wt at aa 330- i.e., Cys at 330 is present
Proper citation: RRID:Addgene_24441 Copy
Species: Mus musculus
Genetic Insert: BAF170
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pRRL-sin18PPT CAG-ires-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19279220
Proper citation: RRID:Addgene_24562 Copy
Species: Mus musculus
Genetic Insert: BAF155
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pRRL-sin18PPT CAG-ires-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19279220
Proper citation: RRID:Addgene_24561 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17013387
Proper citation: RRID:Addgene_24454 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17013387
Proper citation: RRID:Addgene_24451 Copy
Species: Homo sapiens
Genetic Insert: mir-20
Vector Backbone Description: Backbone Size:7651; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20008935
Proper citation: RRID:Addgene_24709 Copy
Species: Aequoria victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5695; Vector Backbone:pUNIV; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17146677
Proper citation: RRID:Addgene_24706 Copy
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