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http://www.addgene.org/24433

Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009

Proper citation: RRID:Addgene_24433 Copy   

•   Source: Addgene

http://www.addgene.org/24434

Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009

Proper citation: RRID:Addgene_24434 Copy   

•   Source: Addgene

http://www.addgene.org/24554

Vector Backbone Description: Backbone Size:4512; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808

Proper citation: RRID:Addgene_24554 Copy   

•   Source: Addgene

http://www.addgene.org/24551

Genetic Insert: Sleeping Beauty
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3712; Vector Backbone:pCMVbeta; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390559

Proper citation: RRID:Addgene_24551 Copy   

•   Source: Addgene

http://www.addgene.org/24430

Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009

Proper citation: RRID:Addgene_24430 Copy   

•   Source: Addgene

http://www.addgene.org/24523

Species: Homo sapiens
Genetic Insert: CLASP2 (340-1362) 9xS/A
Vector Backbone Description: Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for. Due to difficulty in sequencing this plasmid, the EGFP and C-terminus of CLASP2 has not been sequence verified. EGFP-CLASP2 340-1362 9xS/A was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1. AdEasy is a registered trademark of the Johns Hopkins University.

Proper citation: RRID:Addgene_24523 Copy   

•   Source: Addgene

http://www.addgene.org/24486

Species: Mus musculus
Genetic Insert: Long chain acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611

Proper citation: RRID:Addgene_24486 Copy   

•   Source: Addgene

http://www.addgene.org/24522

Species: Homo sapiens
Genetic Insert: CLASP2 (340-1362)
Vector Backbone Description: Backbone Marker:Stratagene, ATCC; Backbone Size:33414; Vector Backbone:pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Comments: See the attached file for the old NIH reference sequence. Numbering starts at amino acid 78 and is off by 8 amino acids because of an insertion that is not accounted for. EGFP-CLASP2 340-1362 was cloned into pShuttle-CMV between KpnI and NotI then recombined by homologous recombination into pAdEasy-1. AdEasy is a registered trademark of the Johns Hopkins University.

Proper citation: RRID:Addgene_24522 Copy   

•   Source: Addgene

http://www.addgene.org/24488

Species: Mus musculus
Genetic Insert: Long chain acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611

Proper citation: RRID:Addgene_24488 Copy   

•   Source: Addgene

http://www.addgene.org/24482

Species: Homo sapiens
Genetic Insert: Sirtuin 4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611

Proper citation: RRID:Addgene_24482 Copy   

•   Source: Addgene

http://www.addgene.org/24419

Species: Homo sapiens
Genetic Insert: RS1
Vector Backbone Description: Backbone Marker:Plasmid 21714: pROSA26-1 (Soriano Lab); Backbone Size:4486; Vector Backbone:pRosa26; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21375737
Comments: Rosa26 targeting vector containing the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences. Plasmid also known as Exp-R26(EF1α-tTA/TetO-mCh-Rs1).

Proper citation: RRID:Addgene_24419 Copy   

•   Source: Addgene

http://www.addgene.org/24536

Species: Synthetic
Genetic Insert: ECFP-IETD2x-Venus
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19053173
Comments: Initiator caspase reporter protein (IC-RP) carries tandem copies of IETD in its linker, a sequence that is efficiently cleaved by caspase-8, but poorly by caspases-3 and -7. IETD constitutes the site in procaspase-3 for initiator caspase cleavage, and IC-RP cleavage is therefore a good readout of procaspase-3 activation. IC-RP was constructed by ligating Venus(YFP) between BamHI and EcoRI sites of pECFP-C1 and ligating linkers encoding cleavage sequences as BspEI-BamHI fragments between ECFP and Venus. Multiple serine and glycine residues flanking the cleavage sequences were added to increase linker flexibility and substrate accessibility. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information

Proper citation: RRID:Addgene_24536 Copy   

•   Source: Addgene

http://www.addgene.org/24498

Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8863834
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_24498 Copy   

•   Source: Addgene

http://www.addgene.org/24530

Species: Homo sapiens
Genetic Insert: Bim L(S44D)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12591950

Proper citation: RRID:Addgene_24530 Copy   

•   Source: Addgene

http://www.addgene.org/24499

Species: Rattus norvegicus
Genetic Insert: Gs alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8863834
Comments: This is Gs alpha with the C-terminal amino acids changed from Gs alpha to Gq alpha residues (QYELL to EYNLV). This construct allows some Gq-coupled receptors to stimulate Adenylate cyclase. There isn't much experience with this chimera at the moment. It may be useful for people who find the AC stimulation is better readout of receptor activation than PLC stimulation. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.

Proper citation: RRID:Addgene_24499 Copy   

•   Source: Addgene

http://www.addgene.org/24490

Species: Mus musculus
Genetic Insert: Long chain acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611

Proper citation: RRID:Addgene_24490 Copy   

•   Source: Addgene

http://www.addgene.org/24492

Species: Homo sapiens
Genetic Insert: Human Sirtuin 3 catalytically inactive mutant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611
Comments: SIRT3 contains a mutation at amino acid Q32H. This mutation is present in the first 100 amino acids of SIRT3 which serve as its mitochondrial import signal. A peptide corresponding to the first 100 aa of SIRT3 is normally cleaved once the protein has been imported into the mitochondria and the active form of SIRT3 in the mitochondrial matrix therefore lacks this part of the protein. Therefore the mutation should not modify SIRT3 activity.

Proper citation: RRID:Addgene_24492 Copy   

•   Source: Addgene

http://www.addgene.org/24491

Species: Homo sapiens
Genetic Insert: Long chain acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5700; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611

Proper citation: RRID:Addgene_24491 Copy   

•   Source: Addgene

http://www.addgene.org/24509

Species: Vibrio fischeri
Genetic Insert: ECFP, EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6724; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note: This plasmid has been partially sequenced. Due to the presence of repeated features in the plasmid that prohibit thorough sequencing, the provided sequence should be considered theoretical and could contain errors. Please refer to Lu, Jian-Ping, Beatty, Laura, and Pinthus, Jehonathan . Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells. Available from Nature Precedings https://www.nature.com/articles/npre.2008.1550.2

Proper citation: RRID:Addgene_24509 Copy   

•   Source: Addgene

http://www.addgene.org/24505

Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRBUBR1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18566452
Comments: Plasmid created by digesting pRBUBR1 with Sph I and ligate the vector backbone together to remove UBR1ha gene. ADH promoter is still present.

Proper citation: RRID:Addgene_24505 Copy   

•   Source: Addgene