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Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The BamHI/BglI fragment from pUAST (Brand and Perrimon, 1993), containing 5 copies
of a GAL4 binding site and the Drosophila hsp70 minimal promoter was subcloned into BamHI site
of pBluescript containing an optimized GFP split with an intron (GG) (Zong et al., 2005). This
strategy generates a hybrid BglII/BamHI site in front of the GFP.
Proper citation: RRID:Addgene_24342 Copy
Genetic Insert: FLPo
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: Mammalian codon-optimized FLP recombinase (FLPo; Addgene plasmid 13792) (Raymond and Soriano, 2007) was PCR amplified to include EcoRI/XbaI restriction sites, and cloned into pQUAS-DSCP to generate pQUAS-DSCP-FLPo.
Proper citation: RRID:Addgene_24358 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pQUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The nuclear LacZ insert was PCR amplified from UAS-nucLacZ genomic flies (Bloomington Stock Center). The KpnI/XbaI digested nucLacZ PCR fragment was then
ligated into pQUAST.
Proper citation: RRID:Addgene_24356 Copy
Species: Neuropspora crassa
Genetic Insert: QF
Vector Backbone Description: Backbone Size:18000; Vector Backbone:pBAC-GH146; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: pBAC-3xPDsRed-GH146-MCS with FseI-AscI-AvrII multi-cloning site has been
previously described (Hong et al., 2009). QF cDNA with 5’ FseI and 3’ Avr restriction sites was
amplified from pAC-QF by PCR, and cloned into the FseI/AvrII restriction sites of pBAC-GH146 to
yield pBac-GH146-QF.
Proper citation: RRID:Addgene_24353 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19289791
Proper citation: RRID:Addgene_24327 Copy
Species: Neurospora crassa/other
Genetic Insert: 5xQUAS/GFP split with an intron (GG)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: This plasmid contains 5 copies of naturally occurring QF binding sites (each 16 bp
long, shown in capital letters, with spacer sequences in small letters):
GGGTAATCGCTTATCCtcGGATAAACAATTATCCtcacGGGTAATCGCTTATCCgctcGGGTAATCG
CTTATCCtcGGGTAATCGCTTATCCtt
This sequence was assembled from overlapping primers and cloned using XhoI and HindIII into
pBluescript containing hsp70 minimal promoter (Brand and Perrimon, 1993) and an optimized GFP
split with an intron (GG) (Zong et al., 2005)
Proper citation: RRID:Addgene_24336 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20434990
Comments: The XhoI/NcoI fragment containing QUAS-Pmin was subcloned from pQUAS-GG
into pGL4.23 (Promega) to replace its minimal promoter. pGL4.23 contains synthetic firefly
luciferase gene (luc2), which has been codon optimized for high expression in mammalian cells.
Proper citation: RRID:Addgene_24337 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: ins-Cre_cryaa-Venus was generated by placing the Cre coding sequence downstream of 1.5 kb of the proximal insulin promoter. The cryaa-Venus cassette was inserted downstream of Cre in the reverse orientation using 0.54 kb of the cryaa promoter.
Proper citation: RRID:Addgene_24333 Copy
Species: Danio rerio
Genetic Insert: H2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19706417
Comments: This plasmid was constructed using 1.5 kb of the hsp70l promoter. The floxed mCherry-STOP and H2b_GFP fragments were PCR amplified individually and sequentially cloned downstream of the hsp70l promoter. The mCherry-STOP cassette serves as a heat-shock induction marker prevents read-through translation of the H2B-GFP fusion protein, which is only expressed in cells that have undergone a Cre mediated excision event. cryaa-Cerulean was inserted downstream of HSB-GFP in the reverse orientation.
*H2B has D26G and V119I mutations. According to the depositor, GFP expression from this construct was always nuclear so there is no reason to suspect that the mutations affect the function (nuclear localization) of the H2B fragment.
Proper citation: RRID:Addgene_24334 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:20350301
Comments: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA]
Proper citation: RRID:Addgene_24545 Copy
Species: Homo sapiens
Genetic Insert: transcription factor Sp3
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.
Proper citation: RRID:Addgene_24541 Copy
Genetic Insert: pN3-Control
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pN3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15247228
Comments: vector control for pN3-Sp1FL and pN3-Sp3FL vectors
Proper citation: RRID:Addgene_24544 Copy
Species: Homo sapiens
Genetic Insert: Sp1FL
Vector Backbone Description: Backbone Marker:Guntram Suske, Addgene plasmid 24544; Backbone Size:4000; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pN3-Control vector also available from Addgene
2 nucleotide mismatches with author sequence detected in the most 3´-UTR. They do not affect the coding sequence of Sp1.
Proper citation: RRID:Addgene_24543 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24439 Copy
Species: A. victoria
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:4557; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24557 Copy
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Comments: TAP tag is 6X his-TEV cleavage site-3X FLAG tag
Proper citation: RRID:Addgene_24556 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Size:4501; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Comments: Also see the following article:
Ofd1, a human disease gene, regulates the length and distal structure of centrioles.
Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF.
Dev Cell. 2010 Mar 16;18(3):410-24.PMID: 20230748
Proper citation: RRID:Addgene_24559 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3893; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24553 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11086009
Proper citation: RRID:Addgene_24432 Copy
Genetic Insert: Sleeping Beauty delta DDE
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3712; Vector Backbone:pCMVbeta; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390559
Proper citation: RRID:Addgene_24552 Copy
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