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Species: Danio rerio
Genetic Insert: vegf
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110173
Proper citation: RRID:Addgene_22417 Copy
Genetic Insert: sh Ataxia telangiectasia mutated
Vector Backbone Description: Backbone Size:8459; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: shATM #2 was used successfully in Rodier et al.(2009) Nat.Cell Biol. 11(8):973-979.
Proper citation: RRID:Addgene_22532 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Kedes Lab; Backbone Size:10200; Vector Backbone:pHBetaAPr-1-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3261634
Comments: The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector.
Proper citation: RRID:Addgene_22533 Copy
Species: Homo sapiens
Genetic Insert: Kif1bβ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5362; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18334619
Proper citation: RRID:Addgene_22534 Copy
Species: Homo sapiens
Genetic Insert: hypoxia inducible factor 1 alpha
Vector Backbone Description: Backbone Size:6262; Vector Backbone:pCAG IGd2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19131628
Proper citation: RRID:Addgene_22535 Copy
Genetic Insert: WT eNOS
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17170139
Proper citation: RRID:Addgene_22497 Copy
Genetic Insert: sh Enhanced Green Fluorescent Protein #1
Vector Backbone Description: Backbone Size:5596; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_22530 Copy
Species: Homo sapiens
Genetic Insert: Codon optimised wild type Calcineurin B
Vector Backbone Description: Backbone Size:7678; Vector Backbone:SFG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19770360
Proper citation: RRID:Addgene_22492 Copy
Species: Homo sapiens
Genetic Insert: Codon optimised Calcineurin A mutant 12
Vector Backbone Description: Backbone Size:7678; Vector Backbone:SFG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19770360
Proper citation: RRID:Addgene_22490 Copy
Species: Mus musculus
Genetic Insert: NEMO shRNA1
Vector Backbone Description: Backbone Marker:Available at Addgene (#12084); Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19847165
Comments: Mouse NEMO shRNA1 in pSicoReverse (Puromycin)
forward oligo:
TGGACATGCTGGGTGAAGAATTCAAGAGATTCTTCACCCAGCATGTCCTTTTTTC
reverse oligo :
TCGAGAAAAAAGGACATGCTGGGTGAAGAATCTCTTGAATTCTTCACCCAGCATGTCCA
Proper citation: RRID:Addgene_22505 Copy
Species: Homo sapiens
Genetic Insert: cytochrome c
Vector Backbone Description: Backbone Marker:PMID 9558351); Backbone Size:5279; Vector Backbone:pBCYC1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14680826
Comments: For an explanation of the cytochrome c expression system, see "Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition." by Pollock et al. PMID 9558351
Proper citation: RRID:Addgene_22468 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pDONRP2r-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17948311
Comments: This clone was amplified with an attB2 site, at the 5’ end and a attB3 site on the 3’ end. The product was then recombined with the pDNR P2R-P3 vector.
Can be used to make 3’ end (C-Term) tagged fusion construct.
Proper citation: RRID:Addgene_22462 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pDONR; Vector Types:Entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17948311
Comments: Egfp ORF was amplified with an attB1 site on the 5’ end and an attB2 site on the 3’ end. ORF is in frame with 3’ entry clone.
Egfp ORF missing the termination codon.
Proper citation: RRID:Addgene_22464 Copy
Genetic Insert: EGFP-mCherry
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCS2+; Vector Types:Injection construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17948311
Comments: pEntregfp3, p3Emcherry, and pCSDest2 were combined in a multisite reaction to create an expression clone where egfp (minus stop codon) is fused in frame with a C terminal mcherry tag
Proper citation: RRID:Addgene_22465 Copy
Species: Merkel Cell Polyomavirus
Genetic Insert: MCPyV VP1
Vector Backbone Description: Backbone Size:5338; Vector Backbone:pGwf; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:19750217
Comments: First reference to this plasmid was in:
Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS. Int J Cancer. 2009 Sep 15.125(6):1250-6
Genbank style annotations can be found at:
http://home.ccr.cancer.gov/LCO/packaging.htm
Proper citation: RRID:Addgene_22515 Copy
Species: Discosoma sp
Genetic Insert: tandem dimer tomato
Vector Backbone Description: Backbone Marker:I. Verma, Salk; Backbone Size:9165; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18162542
Comments: pFUGW-tdtomato
Proper citation: RRID:Addgene_22478 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::mCherry (w introns w/o stop)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2646; Vector Backbone:pDONRP4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_22513 Copy
Species: Mus musculus
Genetic Insert: PIF6APB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5369; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22474 Copy
Species: Mus musculus
Genetic Insert: PhyB NT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5369; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19749742
Proper citation: RRID:Addgene_22471 Copy
Species: Canis lupus familiaris
Genetic Insert: Caveolin 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9325253
Proper citation: RRID:Addgene_22448 Copy
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