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Species: Synthetic
Genetic Insert: GAL4-NIR-FbLAG30
Vector Backbone Description: Backbone Marker:Addgene #184684; Backbone Size:5006; Vector Backbone:pNIR-FbGFPY58/C-JIP; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39242569
Comments: GenBank: MW627294.1 (part of the insert). To make pGAL-NIR-FbLAG30, GAL4 was amplified from a pQP-T2A plasmid (Addgene #102583) and inserted into a pcDNA3.1 plasmid, containing the 15 aa-long linker (Gly3SerGly4SerGly3SerGly2) (Addgene #184684) using KpnI/BamHI restriction enzymes, and NIR-FbLAG30 was inserted using NotI/XbaI restriction enzymes.
Proper citation: RRID:Addgene_220746 Copy
Species: Synthetic
Genetic Insert: NIR-FbLAG30
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5410; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39242569
Comments: GenBank: MW627294.1 (part of the insert). To make pET-NIR-FbLAG30, NIR-FbLAG30 was amplified and inserted to pET22b(+) using NdeI/BamHI restriction enzymes. For cytoplasmic expression pelB signaling sequence (AAATACCTG...ATGGCCATG) was deleted by PCR with a forward primer.
Proper citation: RRID:Addgene_220748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 BA.2.86 RBD
Vector Backbone Description: Vector Backbone:pETcon; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39310091
Proper citation: RRID:Addgene_222231 Copy
Species: Synthetic
Genetic Insert: Tn10-CP25-GFP
Vector Backbone Description: Vector Backbone:pMMK810; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37726481
Comments: Please see Alker et al. 2023 mBio (https://doi.org/10.1128/mbio.01502-23) for more information on the Marine Modification Kit plasmids.
Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539906v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_207138 Copy
Vector Backbone Description: Vector Backbone:pMpGWBs00; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:38824404
Proper citation: RRID:Addgene_222478 Copy
Species: Synthetic
Genetic Insert: LCB3_v1.2
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194228 Copy
Species: Homo sapiens
Genetic Insert: EF1a Promoter
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194225 Copy
Species: Synthetic
Genetic Insert: PGK-ACE2-SynNotch-T2A-mCherry
Vector Backbone Description: Vector Backbone:pAN2414 Lentiviral Destination; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194226 Copy
Species: Synthetic
Genetic Insert: LCB1_v1.3
Vector Backbone Description: Vector Backbone:pMTK0_027 Entry Vector; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194227 Copy
Vector Backbone Description: Vector Backbone:pAN2414 Lentiviral Destination; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194220 Copy
Vector Backbone Description: Vector Backbone:pAN2424 Lentiviral Destination; Vector Types:Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194221 Copy
Species: Caenorhabditis elegans
Genetic Insert: sax-3
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_223463 Copy
Species: Synthetic
Genetic Insert: Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
Vector Backbone Description: Vector Backbone:pSIN-WP-mPGK-GDNF; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27746349
Proper citation: RRID:Addgene_225488 Copy
Species: Caenorhabditis elegans
Genetic Insert: GSPD-1(D60N)
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34532700
Proper citation: RRID:Addgene_224154 Copy
Species: Mus musculus
Genetic Insert: Igκ leader and TAT peptide
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11651, PIs Patrick Aebischer and Didier Trono; Backbone Size:11497; Vector Backbone:pLVUT-tTR-KRAB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27746349
Proper citation: RRID:Addgene_225486 Copy
Species: Caenorhabditis elegans
Genetic Insert: ser-2(prom3)
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_223465 Copy
Species: Synthetic
Genetic Insert: NIR-FbLAG30
Vector Backbone Description: Backbone Marker:Addgene #99123; Backbone Size:5171; Vector Backbone:pAAV-CAG-mRuby2; Vector Types:AAV, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39242569
Comments: GenBank: MW627294.1 (part of the insert). To make pAAV-CAG-NIR-FbLAG30, NIR-FbLAG30 was amplified and swapped with mRuby2 in pAAV-CAG-mRuby2 (Addgene #99123) using KpnI/EcoRI restriction enzymes.
Proper citation: RRID:Addgene_220750 Copy
Species: Synthetic
Genetic Insert: EF1a-LCB1-PDGFR TMD-T2A-mCherry
Vector Backbone Description: Vector Backbone:pAN2414 Lentiviral Destination; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194231 Copy
Species: Synthetic
Genetic Insert: EF1a-LCB3-PDGFR TMD-T2A-mCherry
Vector Backbone Description: Vector Backbone:pAN2414 Lentiviral Destination; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194232 Copy
Species: Synthetic
Genetic Insert: PGK-LCB3-SynNotch-T2A-mCherry
Vector Backbone Description: Vector Backbone:pAN2414 Lentiviral Destination; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33907743
Comments: Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
Proper citation: RRID:Addgene_194230 Copy
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