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Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153234 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134768 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141300 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141303 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141305 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:14079; Vector Backbone:pRATIO1267; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141312 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:14000; Vector Backbone:pRATIO2212; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141313 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11322; Vector Backbone:pGWB701; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141288 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8002; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35254 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8328; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35252 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8410; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35251 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7877; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35246 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7647; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
https://www.jic.ac.uk/research-impact/technology-platforms/genomic-services/crop-transformation/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35245 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9276; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35249 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9528; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35248 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: attP Site
Vector Backbone Description: Vector Backbone:pAM2314; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: our engineered NS1 Gateway “donor vector”
the attP region of pDONR221 was amplified with primers H3140 and H3141, incorporating MluI and XhoI sites respectively and cloned into the NS1 vector pAM2314. the attP amplified region contains the upstream and downstream M13F and M13R annealing sites for sequencing inserts
Proper citation: RRID:Addgene_40247 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:6005; Vector Backbone:pGreen III; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_51777 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_48257 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_48255 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRT100/pPZP312; Vector Types:Binary Gateway vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_48256 Copy
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