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Species: Homo sapiens
Genetic Insert: YTHDF1 (262-559)
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32451507
Proper citation: RRID:Addgene_155349 Copy
Species: Synthetic
Genetic Insert: humanized EBpaRS/BstYam
Vector Backbone Description: Backbone Marker:Clontech, major modifications; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929862
Comments: The plasmid has been developed for efficient incorporation of Bpa in mammalian cells, first reported in Böttke et al, EMBO reports, 2020. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EBpaRS and 4 copies of the U6-BstYam tRNA cassette downstream of the synthetase cassette.
Proper citation: RRID:Addgene_155342 Copy
Species: Homo sapiens
Genetic Insert: Citron
Vector Backbone Description: Vector Backbone:Addgene #12247; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155345 Copy
Species: Yarrowia lipolytica
Genetic Insert: tef1 promoter - xylulose kinase - pex20 terminator
Vector Backbone Description: Backbone Size:2833; Vector Backbone:pCfB6630; Vector Types:Yeast Expression, EasyCloneYALI insert vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33685391
Comments: Please note that there are a few differences between the Addgene NGS result and depositor's sequence. These discrepancies do not affect plasmid function.
Proper citation: RRID:Addgene_155341 Copy
Species: Bos taurus
Genetic Insert: RAP1B
Vector Backbone Description: Backbone Marker:Campeau lab (addgene #19068); Backbone Size:9605; Vector Backbone:pLenti PGK Puro DEST (w529-2); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Comments: flag-RAP1B-V12 cDNA was amplified by PCR and cloned into pENTR DTOPO and transferred by Gateway cloning into Desination vector. bioRxiv 10.1101/792077
Proper citation: RRID:Addgene_156168 Copy
Species: S. sonneii
Genetic Insert: rRNA operon, orthogonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156322 Copy
Species: E. coli
Genetic Insert: rRNA operon, orthogonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156324 Copy
Species: Mus musculus
Genetic Insert: Thrombospondin-1
Vector Backbone Description: Backbone Size:3601; Vector Backbone:Modified pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31255486
Proper citation: RRID:Addgene_156162 Copy
Species: Mus musculus
Genetic Insert: Tg-M71(161,176)-LacZpA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21111823
Proper citation: RRID:Addgene_15605 Copy
Species: Mus musculus
Genetic Insert: Tg-MOR23(148, 40)-LacZpA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS; Vector Types:high copy number; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21111823
Proper citation: RRID:Addgene_15604 Copy
Species: Homo sapiens
Genetic Insert: SLC38A2
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLenti CMV Blast DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32341021
Comments: Silent mutations are as follows: aat ctg agc aat gcg att gtg ggc agt --> AAC TTG TCC AAC GCA ATC GTT GGT TCT.
Proper citation: RRID:Addgene_156180 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8633.
Proper citation: RRID:Addgene_15600 Copy
Species: A. faecalis
Genetic Insert: rRNA operon, orthagonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156339 Copy
Species: P. stuartii
Genetic Insert: rRNA operon, orthogonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156333 Copy
Species: Homo sapiens
Genetic Insert: RAP2A
Vector Backbone Description: Backbone Marker:Campeau lab (addgene #19067); Backbone Size:9778; Vector Backbone:pLenti PGK Neo DEST (w531-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Comments: RAP2A-V12 cDNA was amplified by PCR and cloned into pENTR DTOPO and transferred by Gateway cloning into Desination vector. bioRxiv 10.1101/792077
Proper citation: RRID:Addgene_156178 Copy
Species: P. mirabilis
Genetic Insert: rRNA operon, orthogonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156332 Copy
Species: Homo sapiens
Genetic Insert: RAP2A
Vector Backbone Description: Backbone Marker:Campeau lab (addgene #19067); Backbone Size:9778; Vector Backbone:pLenti PGK Neo DEST (w531-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Comments: RAP2A-WT DNA fragment was cloned into pENTR DTOPO and transferred by Gateway cloning into Desination vector. bioRxiv 10.1101/792077
Proper citation: RRID:Addgene_156177 Copy
Species: Bos taurus
Genetic Insert: RAP1B
Vector Backbone Description: Backbone Marker:Campeau lab (addgene #19067); Backbone Size:9778; Vector Backbone:pLenti PGK Neo DEST (w531-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Comments: flag-RAP1B-N17 cDNA was amplified by PCR and cloned into pENTR DTOPO and transferred by Gateway cloning into Desination vector. bioRxiv 10.1101/792077
Proper citation: RRID:Addgene_156176 Copy
Species: E. cloacae
Genetic Insert: rRNA operon, orthogonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156330 Copy
Species: Homo sapiens
Genetic Insert: RAP2A
Vector Backbone Description: Backbone Marker:Campeau lab (addgene #19068); Backbone Size:9605; Vector Backbone:pLenti PGK Puro DEST (w529-2); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Comments: RAP2A-V12 cDNA was amplified by PCR and cloned into pENTR DTOPO and transferred by Gateway cloning into Desination vector. bioRxiv 10.1101/792077
Proper citation: RRID:Addgene_156171 Copy
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